Effect of Panax Notoginseng Saponins on EMT of Rat Renal Proximal Tubular Epithelial Cells Induced by TGF-β1
10.13422/j.cnki.syfjx.20190636
- VernacularTitle: 三七皂苷对TGF-β1诱导的大鼠肾小管上皮细胞EMT的影响
- Author:
Jing PAN
1
;
Yue-guang DU
1
;
Yi GUO
1
Author Information
1. College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China
- Publication Type:Research Article
- Keywords:
panax notoginseng saponins;
transforming growth factor-β1(TGF-β1);
silent information regulation 2 homolog 1(SIRT1)/TGF-β1/Smad pathway;
epithelial-mesenchymal transition(EMT);
diabetic nephropathy
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):89-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the intervention effect of panax notoginseng saponins (PNS) on epithelial-mesenchymal transition (EMT) of rat renal tubular epithelial cells (NRK-52E) induced by transforming growth factor-β1(TGF-β1), and analyze the mechanism based on the silent information regulation 2 homolog 1(SIRT1)/TGF-β1/Smad signaling pathway. Method:NRK-52E were cultured in DMEM medium with 10% fetal bovine serum, and divided into normal control group, TGF-β1 group (5 μg·L-1), resveratrol (RSV) group (50 mg·L-1), EX527 group (10 μmol·L-1), Panax notoginseng saponins (PNS) group (100 mg·L-1), and EX527+ PNS group (10 μmol·L-1+100 mg·L-1). Then cells were collected after drug intervention for 48 h. The expressions of α-SMA,E-cadherin,SIRT1,TGF-β1,Smad3,Smad4 mRNA in each group were detected by Real-time PCR. The protein expressions of α-SMA, E-cadherin,SIRT1 and TGF-β1 were detected by Western blot. Result:Compared with normal group, mRNA and protein expressions of α-SMA increased obviously(P<0.05), but E-cadherin decreased significantly(P<0.01)in TGF-β1 group. Compared with TGF-β1 group, mRNA and protein expressions of α-SMA decreased significantly(P<0.01), while E-cadherin increased(P<0.01)in resveratrol and PNS groups, and EMT was inhibited. Meanwhile, mRNA and protein expressions of SIRT1 increased significantly(P<0.01), while mRNA expressions of TGF-β1,Smad3,and Smad4 decreased(P<0.01). Under the intervention of SIRT1 blocker EX527, PNS could not play a significant inhibitory effect on the cells. Conclusion:PNS can prevent the occurrence of EMT of renal tubular epithelial cells induced by TGF-β1, and the mechanism may be related to active SIRT1 to inhibit TGF-β1/Smad pathway.
