Effect of Wendantang on Related Inflammatory Factors and Expression of STAT3 as a Key Molecule in JAK2/STAT3 Pathway of Rats with Obesity Phlegm-dampness Syndrome
10.13422/j.cnki.syfjx.20190448
- VernacularTitle: 温胆汤对肥胖痰湿证大鼠相关炎症因子及JAK2/STAT3通路关键分子STAT3表达的影响
- Author:
Song-ren YU
1
;
Qing SHU
1
;
Yang BAI
1
;
Qi YAO
1
;
He-bao WANG
1
;
Li ZHOU
1
;
Ping WANG
1
;
Shao-min CHENG
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
obesity with syndrome of phlegm-dampness;
Wendantang;
inflammatory factors;
signal transducer and activator of transcription 3(STAT3);
Janus kinase 2/STAT3(JAK2/STAT3) pathway;
Lee's index;
chronic inflammation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):39-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Wendantang on tumor necrosis factor-α (TNF-α),interleukin(IL)-6,IL-17,IL-22 and other related inflammatory factors in peripheral blood and the expression of STAT3[the key molecule of Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3) signal pathway in hypothalamus] mRNA and protein of obese rats with syndrome of phlegm-dampness,so as to explore the internal mechanism of Wendantang in interfering obesity with syndrome of phlegm-dampness. Method:A total of 100 rats were randomly divided into blank group(30 rats) and modeling group(70 rats),rats in the blank group was fed with basic feed and the modeling group was fed with high-fat feed for 6 weeks.Animal model of obesity with syndrome of phlegm-dampness was established by the method in literature.After successful modeling,16 obese rats were selected and randomly divided into the model group and Wendantang intervention group with 8 rats in each group,and another 8 rats in the blank group were randomly selected as the normal group.Rats in Wendantang intervention group were given 15 g·kg-1 of crude drug by gavage,while the model group and the normal group were given the same amount of distilled water for gavage once a day for 6 weeks.No eating but no prohibiting drinking before dealing with 12 h and then taking samples after anesthesia.The body weight,Lee's index and obesity rate of rats were measured,the levels of blood lipids[total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C)] of rats were detected with a full-automatic biochemical analyzer according to the requirements of the kit,the expression of TNF-α,IL-17,IL-22 and IL-6 in peripheral blood of rats was detected with enzyme-linked immunosorbent assay(ELISA),STAT3 mRNA expression in hypothalamus of rats was detected with real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) and the expression of STAT3 protein in hypothalamus of rats was detected with Western blot. Result:The high-fat feed feeding could successfully replicate the obese rat model,and the obesity rate of rats in the modeling group was greater than 20%,and compared with the blank group,the body weight and Lee's index of rats in the modeling group were significantly increased(P<0.05,P<0.01).Compared with the normal group,the body weight of rats in the model group was significantly increased(P<0.01),blood lipid level changed greatly(P<0.01),levels of related inflammatory factors were significantly increased(P<0.01),the STAT3 mRNA and protein expression in hypothalamus tissue of rats was significantly increased(P<0.01).Compared with the model group,the body weight and Lee's index of rats in Wendantang intervention group significantly decreased(P<0.05,P<0.01),blood lipid level significantly changed(P<0.01),levels of related inflammatory factors were significantly decreased(P<0.01),the STAT3 mRNA and protein expression in hypothalamus tissue of rats in Wendantang intervention group was significantly decreased(P<0.01). Conclusion:Wendantang has a good effect on improving phlegm-dampness in obese rats,and the mechanism may be related to regulating JAK2/STAT3 signal pathway then to improve the chronic inflammatory state of the body,and all of which provides a scientific basis for Wendantang in intervening obesity with syndrome of phlegm-dampness.
