Effect of Recipes for Tonifying Kidney and Replenishing Qi on Learning and Memory-related Genes in Aged Rats
10.13422/j.cnki.syfjx.20190601
- VernacularTitle: 补肾益气方药左归丸和益气聪明汤对老年大鼠学习记忆能力及相关基因表达的影响
- Author:
Lu WANG
1
;
Li-e SUN
1
;
Xiang-ping KANG
1
;
Xue-li ZHANG
1
;
Hai-yan HU
1
;
Zhang-bin GONG
1
;
Guo-qin JIN
1
Author Information
1. Basic Medical School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
aging;
tonifying kidney decoction;
Zuoguiwan;
Yiqi decoction;
Yiqi Congming Tang;
learning and memory;
epigenetic modification
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):15-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of recipes for tonifying kidney and replenishing Qi, Zuoguiwan (ZG) and Yiqi Congming Tang(YQ) on memory capacity, expressions of learning and memory-related genes expression, and explore the changes in relevant epigenetic modification enzymes. Method:SD male rats with natural aging (24 months old) were used as animal models and randomly divided into aged control group, aged ZG group(12.12 g·kg-1), aged YQ group(10.18 g·kg-1), aged compound group(11.15 g·kg-1) and aged antagonist RU38486 group(5×10-3g·kg-1). Another 5 months old male SD rats were included as the young control group. Morris water maze method was used to observe the spatial learning and memory ability of the rats. The co-localizations of histone deacetylase 2 (HDAC2) and methyl CpG binding protein 2 (MeCP2) in hippocampus of rats in each group were observed by laser confocal microscope. The changes in expressions of glucocorticoid receptor (GR), synapsin1(Syn-1), HDAC2, and histone acetyltransferase 1(HAT1) proteins in hippocampus of each group were detected by Western blot, and mRNA expression of HDAC2 was detected by Real-time fluorescence quantitative polymerase Chain reaction (Real-time PCR). At the same time, the effects of ZG, YQ and compound decoction in alleviating the above-mentioned abnormal changes were observed. Result:Compared with the young control group(control group), the latency of the aged control group was significantly prolonged (P<0.05), the number of crossing platforms was significantly reduced (P<0.05); compared with the aged control group(model group), the latencies of the ZG group and the YQ group were significantly shortened (P<0.05), and the numbers of crossing platforms were increased. Compared with the young control group, the protein and mRNA expressions of HDAC2 in the aged control group were significantly increased (P<0.05,P<0.01), and the co-expression with MeCP2 in the nucleus was significantly increased; and the expressions of HAT1, GR and Syn-1 were significantly decreased(P<0.05,P<0.01). ZG, YQ and compound can all alleviate the abnormal changes of the above indicators to varying degrees, but the YQ and the compound group had no effect on HAT1. Conclusion:ZG group, YQ group, and compound group can improve the spatial learning and memory abilities of aged rats by increasing the expression of learning-memory-associated protein GR and epigenetic modification enzyme HAT1, and reducing the expression of HDAC2 and the co-localization of HDAC2 protein and MeCP2 in the nucleus.
