Effect of Zuoguiwan on mRNA Expression of Orexin and Its Receptors in Hypothalamus of Ovariectomized Rats
10.13422/j.cnki.syfjx.20191021
- VernacularTitle: 左归丸对去卵巢大鼠下丘脑Orexin及其受体mRNA表达的影响
- Author:
Su-min YE
1
;
Feng TAN
1
;
Fei-xiang LIU
1
;
Dao-ming XU
2
;
Yi CHAI
1
;
Ming-yue ZHANG
1
;
Qiao-ling FAN
1
Author Information
1. College of Basic Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, China
2. Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China
- Publication Type:Research Article
- Keywords:
Zuoguiwan;
postmenopausal osteoporosis(PMOP);
orexin;
orexin receptor 1 (OX1R);
orexin receptor 2 (OX2R)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(18):9-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the mRNA levels of Orexin and its receptors in the hypothalamus of ovariectomized osteoporosis rats, in order to explore the pathogenesis of postmenopausal osteoporosis(PMOP) and the mechanism Zuoguiwan. Method: An osteoporosis model induced by ovariectomy was established in rats. Totally 32 female Sprague-Dawley (SD) rats were randomly divided into sham-operated group, ovariectomized model group, 17β-estradiol treated positive group, and Zuoguiwan group, with 8 rates in each group. After 12 weeks of intragastric administration, the bone mineral density (BMD) and trabecular microstructural changes of femur were detected by micro-CT (μ-CT), and the morphological changes of bone tissue were observed by hematoxylin-eosin staining (HE) staining. The markers of bone turnover in serum osteocalcin (OCN), N-terminal propeptide of type Ⅰ procollagen (PINP), tartrate-resistant acid phosphatase (TRAP) were measured using enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of orexin, orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R) were measured by Real-time PCR. Result: Compared with sham-operated group, the μ-CT showed that BMD, bone volume fraction(BV/TV), trabecular thickness(Tb. Th)and trabecular number(Tb. N)in ovariectomized model group were significantly decreased (P<0.01), while Trabecular Separation(Tb. Sp) was increased (P<0.01), and the bone trabeculae were obviously sparse, and the spacing was widened. HE staining showed that the trabecular bone was significantly rare, the structure was incomplete, the arrangement was loose, and the spacing was widened. ELISA showed that serum osteocalcin (OCN),and N-terminal propeptide of type Ⅰ procollagen (PINP) levels decreased, whereas tartrate-resistant acid phosphatase (TRAP) content increased (P<0.01), and hypothalamic Orexin, OX1R, OX2R mRNA expressions were down-regulated (P<0.01). After treatment, BMD, BV/TV, Tb. Th, Tb. N in Zuoguiwan group increased (P<0.05,P<0.01), while Tb. Sp decreased (P<0.01),bone trabeculae became dense, void fraction decreased, distribution was uniform, arrangement was acceptable, and the microstructure was significantly improved.serum OCN and PINP increased (P<0.05,P<0.01), while TRAP content decreased (P<0.01), the mRNA expressions of Orexin, OX1R and OX2R in the hypothalamus were significantly up-regulated (P<0.05,P<0.01). Conclusion: Decreased mRNA levels of Orexin and its receptors in the hypothalamus may be one of the mechanisms of PMOP. Zuoguiwan may correct the imbalance of bone metabolism, improve the trabecular microstructure and improve bone by up-regulating the mRNA expressions of Orexin and its receptors in the hypothalamus, density, so as to show a therapeutic effect on PMOP.
