Effect of Puerarin Combined with Tanshinone ⅡA on t-PA, PAI-1 and Oxidative Stress in DM Rats with Vascular Lesions
10.13422/j.cnki.syfjx.20191905
- VernacularTitle: 葛根素联合丹参酮ⅡA对糖尿病血管病变大鼠t-PA，PAI-1及氧化应激的影响
- Author:
Xue YANG
1
;
Fei ZHOU
2
;
Yang YANG
1
;
Chao-li LUO
1
;
Wen-tao HUANG
1
;
Xian-qin LUO
1
;
Xiao-ming ZHONG
2
;
Heng-hua LI
1
Author Information
1. Chongqing Academy of Chinese Material Medica, Chongqing Center for Safety Evaluation of Drugs, Chongqing 400065, China
2. School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China
- Publication Type:Research Article
- Keywords:
puerarin combined with tanshinoneⅡA;
diabetesmellitus;
vascular lesion;
oxidative stress;
tissue plasminogen activator (t-PA);
plasminogen activator inhibitor-1 (PAI-1)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(19):46-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the protective effect and mechanism of combination of puerarin combined with tanshinone ⅡA on diabetes mellitus (DM) rats with vascular lesions. Method:The SD rats (fed with high-fat diet) were administrated with streptozotocin(STZ) through intravenous injection to make the model of diabetic vascular lesions. The successfully modeled rats were randomly divided into the model control group, the high-dose group (0.5 g·kg-1+1.0 g·kg-1), the middle-dose group (0.25 g·kg-1+0.5 g·kg-1), the low-dose group (0.05 g·kg-1+0.1 g·kg-1), the puerarin group (0.25 g·kg-1), the tanshinone ⅡA group (0.5 g·kg-1) and the positive control group (Metformin, 0.09 g·kg-1). Each group was administrated with drugs respectively by gavage for 70 days. After intervention in each group, the general conditions and body weight of the rats were observed. The contents of blood grucose and blood lipids were determined by automatic biochemical analyzer. The contents of insulin, advanced glycation end products (AGEs), superoxide dismutase(SOD), glutathione peroxidase (GSH-Px) in serum, the contents of tissue plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1) and thromboxane B2 (TXB2) in plasma, as well as the contents of AGEs and oxidized low-density lipoprotein(ox-LDL) in aorta homogenate were detected by enzyme-linked immunosorbent assay(ELISA). The content of malondialdehyde(MDA) in serum was determined by chemical colorimetry. Pathological changes of coronary tissue were observed by htoxylin eosin(HE) staining. The expression of PAI-1 protein of aorta was observed by immunohistochemistry. Result:Compared with the normal control group, in the model group, the levels of blood grucose and blood lipids (P<0.05,P<0.01), the contents of insulin, AGEs and MDA in serum (P<0.05, P<0.01), the contents of PAI-1 and TXB2 in plasma (P<0.01), and the contents of AGEs and ox-LDL in aorta homogenate were increased(P<0.01). The contents of SOD and GSH-Px in serum (P<0.01), the contents of t-PA in plasma were decreased(P<0.05), whereas the expression of PAI-1 protein of aorta was increased (P<0.05). Compared with the model group, in the drug intervention group, the levels of blood grucose and blood lipids (P<0.05, P<0.01), the contents of insulin, AGEs and MDA in serum (P<0.05,P<0.01), the contents of PAI-1 and TXB2 in plasma (P<0.05, P<0.01), and the contents of AGEs and ox-LDL in aorta homogenate were decreased(P<0.05,P<0.01), whereas the contents of SOD and GSH-Px in serum and the contents of t-PA in plasma were increased(P<0.05,P<0.01). The coronary lesions were relieved, and the expression of PAI-1 protein of aorta was reduced (P<0.05). The efficacy of combined medication was better than single drug administration. Conclusion:Puerarin combined with Tanshinone ⅡA could relieve vascular lesions of DM rats. The mechanisms may be related to the reduction of oxidative stress and the regulation of coagulation-fibrinolysis system.
