Effect and Mechanism of Pilose Antler Different Components on Bone Tissue of Ovariectomized Osteoporosis Model Rats
10.13422/j.cnki.syfjx.20192003
- VernacularTitle: 鹿茸不同组分对去卵巢骨质疏松症大鼠骨组织的作用及其机制
- Author:
Wei GONG
1
;
Hong-xin ZHENG
1
;
Dong-xiang YANG
2
;
Feng LI
1
;
Lei-ming QU
2
;
Wei LIU
1
Author Information
1. Liaoning University of Traditional Chinese Medicine(TCM), Shenyang 110032, China
2. Affiliated Hospital of Liaoning University of TCM, Shenyang 110032, China
- Publication Type:Research Article
- Keywords:
pilose antler polysaccharides;
pilose antler polypeptide;
osteoporosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(20):36-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of pilose antler different components on the bone tissue of ovariectomized osteoporosis model rats and ascertain the material basis of pilose antler. Method:fifty-six SD rats were divided randomly into seven groups:normal group,model group,Xianling Gubao group(468 mg·kg-1),Bujiale group(80 mg·kg-1),polysaccharide group(50 mg·kg-1),polypeptides group(175 mg·kg-1),polysaccharide and polypeptide mixture group(50 mg·kg-1+175 mg·kg-1). Osteoporosis mode was established through ovary resection of female rats,meanwhile,the rats were given different components of pilose antler for consecutively 12 weeks. Subsequently, using absorptiometry to measure the rats' bone mass density. The activities of bone alkaline phosphatase(BALP),osteocalcin (OT),bone morphogenetic protein2(BMP-2),Smad1,Smad5,Runt-related transcription factor 2 (RUNX2) were detected by enzyme-linked immuno sorbent assay (ELISA). The expression of BMP-2,Smad1,Smad5,Runx2 protein was examined by Western blot and Real-time polymerase chain reaction (Real-time PCR). Morphological assay for bone tissue were detected by htoxylin eosin(HE) staining. Result:After 12 weeks, Compared with the normal group, the osteoporosis model group showed significantly decrease in bone mineral density(P<0.05), and bone BALP,OT,BMP-2,Smad1,Smad5, Runx2 content(P<0.05), the mRNA and protein expression of BMP-2,Smad1, Smad5, Runx2 in bone were significantly decrease(P<0.05). Bone tissue was destroyed. Compared with the osteoporosis model group,different components of pilose antler could significantly improve the activities of BALP,OT,BMP-2,Smad1, Smad5, Runx2 and bone mineral density in bone,and up-regulate the mRNA and protein expression of BMP-2,Smad1,Smad5,Runx2.Bone structure is repaired. Conclusion:Pilose antler different components has therapeutic effect on ovariectomized osteoporosis model rats.The mechanism may be related to up-regulat the expression of BMP-2/Smad1,Smad5/Runx2 signal pathways.
