Suanzaoren Tang Exerts Neuroprotective Effect by Inhibiting Hippocampal Neuroinflammation in APP/PS1 Double Transgenic Mice
10.13422/j.cnki.syfjx.20191936
- VernacularTitle: 酸枣仁汤通过抑制APP/PS1双转基因小鼠海马神经炎症发挥神经保护作用
- Author:
Qing-hua LONG
1
;
Li DING
1
;
Bin-bin ZHAO
1
;
Bo XU
1
;
Ai-hua TAN
1
;
Ping WANG
1
Author Information
1. Institute of Geriatrics, Hubei University of Chinese Medicine, Wuhan 430065, China
- Publication Type:Research Article
- Keywords:
Suanzaoren Tang;
Alzheimer's disease;
neuroinflammation;
neuroprotection;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(20):1-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Suanzaoren Tang on hippocampal neuroinflammation in APP/PS1 mice and to explore its possible mechanism of neuroprotection. Method:The mice were randomly divided into blank group, model group, donepezil group(0.92 mg·kg-1), Suanzaoren Tang low and high-dose groups(12.96,25.92 g·kg-1). After 30 days of continuous administration in each group, pathological changes of dentate gyrus (DG) in hippocampus of mice in each group were observed by Nissl staining.The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in serum of each group were detected by enzyme-linked immunosorbent assay (ELISA). Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the mRNA expression levels of TNF-α and IL-1β in hippocampus of each group. The expression levels of glial fibrillary acidic protein (GFAP) and ionic calcium binding protein 1 (IBA1) in hippocampus of each group were detected by immunohistochemical staining (ICH) and Western blot. Result:Compared with blank group, the granule cells in DG region were unevenly arranged in model group, with obvious cell loss, and the nissl bodies in some neurons disappeared or condensed, serum TNF-α and IL-1β content significantly increased (P<0.01), mice hippocampus of TNF-α and IL-1β mRNA expression quantity significantly increased (P<0.01), levels of GFAP and IBA1 in the hippocampal DG region were significantly up-regulated (P<0.01). Compared with model group,the granule cells in the hippocampal DG region were slightly aligned in donepezil group, Suanzaoren Tang low and high-dose groups, neuron loss of ease, and intracellular austenite disappear or pyknotic conditions improve,the expressions levels of TNF-α and IL-1β in the serum were decreased(P<0.05,P<0.01),the expression levels of TNF-α and IL-1β mRNA in the hippocampus were decreased(P<0.05,P<0.01), protein expression levels of GFAP and IBA1 in the hippocampal DG region were decreased(P<0.05,P<0.01). Conclusion:Suanzaoren Tang can improve neuronal loss in APP/PS1 double transgenic mice, and its mechanism may be related to the regulation of hippocampal neuroinflammation in mice.
