Effect of Biling Qutong Prescription on SIRT3 Protein Expression and URAT1 mRNA in Skeletal Muscle of Diabetic Gout Rats
10.13422/j.cnki.syfjx.20191903
- VernacularTitle: 萆苓祛痛方对糖尿病痛风大鼠骨骼肌组织SIRT3蛋白表达及URAT1 mRNA的影响
- Author:
Zhong-nan LI
1
;
Yan-yang XING
2
;
Yuan-yuan ZHOU
2
;
Shu-hong MA
2
;
Yu-ting XING
2
;
De-mei DOU
2
;
Zhao-hui FANG
1
Author Information
1. The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China
2. Graduate School, Anhui University of Chinese Medicine, Hefei 230038, China
- Publication Type:Research Article
- Keywords:
Biling Qutong prescription;
diabetic gout rats;
skeletal muscle tissue;
sirtuin 3(SIRT3);
urate transporter 1 (URAT1)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(21):25-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of phlegm and blood stasis on the expressions of sirtuin 3(SIRT3)protein and urate transporter 1(URAT1) mRNA in skeletal muscle of diabetic rats with gout. Method: The 40 healthy rats, excepting the normal group, the remaining groups were fed with high-fat diet combined with low-dose streptozotocin solution (40 mg·kg-1) once a day, with blood glucose "16.7 mmol·L-1" as the criterion for the diabetes model. After 4 days, the 5% sodium urate solution was injected into the joint cavity once to induce the gout model. After the successful modeling, the Biling group (10 g·kg-1), the indomethacin group (5 mg·kg-1) and the pioglitazone group (10 mg·kg-1) continued to be administered for 21 days. The normal group and the model group were given the same amount of normal saline. The expression of SIRT3 protein in skeletal muscle tissue was determined by Western blot, URAT1 mRNA expression in bone tissue was detected by quantitative real-time fluorescence polymerase chain reaction(Real-time PCR),and blood was collected to measure blood glucose (GLU), blood uric acid (UA) and C-reactive protein (CRP). Result: Compared with the normal group, GLU, UA and CRP in the model group were significantly increased (P<0.01). Compared with the model group, GLU in the Biling group and the pioglitazone group were decreased, and UA and CRP in the medicine group were significantly decreased (P<0.05,P<0.01). Compared with the normal group, the expression of SIRT3 protein in the skeletal muscle of the model group was significantly decreased (P<0.01), while the expression of SIRT3 protein in the skeletal muscle of the Biling group was significantly increased after administration (P<0.01). Compared with the model group, the expression of SIRT3 protein in the skeletal muscle of the sputum group was significantly increased (P<0.01), with no significant difference from the western medicine group. The results of the strip chart also showed that compared with the model group, the expression brightness of the model group was significantly weakened, while the expression brightness of the drug group was significantly enhanced. Compared with the normal group, the relative expression of joint URAT1 mRNA in the model group was significantly increased (P<0.01). Compared with the model group, the relative expression of URAT1 mRNA in each drug group was significantly down-regulated (P<0.01). The results of the strip chart also showed that expression brightness of the normal group was the weakest, while that of the model group was the highest, and the expression brightness of the Biling group and the western medicine group was significantly weakened. Joint pathology suggested that compared with the normal group, the pathological damage of the joints in the model group was severe, with a large number of inflammatory cell infiltration and fibrosis, synovial cell degeneration and necrosis. Compared with the model group, the degree of joint disease was significantly reduced after treatment with Biling Qutong prescription, with only a small amount of inflammatory cell infiltration and mild hyperplasia in synovial epithelium. Conclusion: Biling Qutong prescription with effects in purging turbidity, detoxifying and dredging collaterals can significantly reduce the content of serum inflammatory factor CRP, significantly increase the protein expression of SIRT3 in skeletal muscle tissue of model rats, lower the content of URAT1 mRNA, reduce the blood glucose and blood uric acid levels in diabetic gout rats, and protect joints.
