Effect of Drug-containing Serum of Clerodendrum bungei on Liver Cancer Cells Through PI3K/Akt Signaling Pathway
10.13422/j.cnki.syfjx.20191222
- VernacularTitle: 臭牡丹含药血清通过PI3K/Akt信号通路对肝癌细胞的影响
- Author:
Qi HU
1
;
Ding-yao ZHU
1
;
Xiao-ning TAN
2
;
Ke-jian ZHU
2
Author Information
1. Hunan University of Chinese Medicine, Changsha 410208, China
2. Hunan Academy of Chinese Medicine Affiliated Hospital, Changsha 410006, China
- Publication Type:Research Article
- Keywords:
Clerodendron bungei;
liver cancer cell MHCC97-H;
phosphatidylinositol 3-kinase(PI3K)/protein kinase (Akt) signaling pathway;
drug-containing serum
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):28-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Clerodendrum bungei-containing serum on liver cancer MHCC97-H cells and its possible mechanism from the perspective of phosphatidylinositol 3-kinase(PI3K)/protein kinase (Akt) signaling pathway. Method: The medicinal serum of 15% C. bungei was used to treat MHCC97-H cells. The effect of serum containing C. bungei on cell proliferation was observed by cell counting kit-8(CCK-8) method, in order to select the best time and concentration. The apoptosis was detected by Annexin V-FITC/PI double staining method. Western blot was used to detect the posphatase and tensin homologous gene deleted from chromosome 10 in key proteins (PTEN), phosphoprotein kinase B (p-Akt) and phosphatidylinositol 3-kinase (PI3K)-related protein expression of PI3K/Akt signaling pathway. Real-time PCR was used to detect C. bungei-containing serum on cells for 72 h after activation of nuclear factor-activated B cell kappa light chain(NF-κB) and tumor necrosis factor-α (TNF-α) mRNA expression. Result: The results of CCK-8 showed an inhibitory effect of the C. bungei-containing serum on the proliferation of tumor cells in a dose and time-dependent manner. Among them, the high-dose group had the most obvious inhibitory effect, and the maximum inhibition rates at 24, 48,72 h were 28%, 32%, and 43%, respectively. The results of flow cytometry showed that with the increase of drug-containing serum concentration, the cell growth was observed. The inhibition rate of cells was increased to different degrees, and the inhibition effect was significantly increased in the 72 h intervention group (P<0.01). The maximum apoptosis rate of the C. bungei-containing serum group was 19.48% and 19.72%, compared with the blank group. The difference was significant (P<0.05). The results of Western blot showed that the expression of PTEN in different groups increased with different concentrations of C. bungei-containing serum (P<0.05), and the expressions of PI3K and p-Akt decreased (P<0.05). Real-time PCR results showed that the C. bungei-containing serum could down-regulate the expression of NF-κB and up-regulate the expression of TNF-α mRNA (P<0.05). Conclusion: The medicinal serum of C. bungei can effectively inhibit the proliferation of MHCC97-H hepatoma cells and promote its apoptosis, which may be related to the PI3K/Akt signaling pathway and its key factors.
