Effect of Hei Xiaoyaosan on Expressions of APP，PERK in Hippocampus Area of Alzheimer's Dementia Mice
10.13422/j.cnki.syfjx.20192101
- VernacularTitle: 黑逍遥散对AD模型小鼠海马区APP，PERK表达的影响
- Author:
Shu-mei CUI
1
;
Hong-yan WU
1
;
Chun-lin MA
1
;
Qing-tao ZENG
2
;
Jia-nan LIU
3
;
Kai-min ZHU
1
Author Information
1. Gansu University of Chinese Medicine, Lanzhou 730000, China
2. Shenzhen Luohu People's Hospital, Shenzhen 518020, China
3. Dingxi Second People's Hospital, Dingxi 743000, China
- Publication Type:Research Article
- Keywords:
Alzheimer's disease;
Hei Xiaoyaosan;
endoplasmic reticulum stress
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Hei Xiaoyaosan on endoplasmic reticulum stress in hippocampal neurons of Alzheimer's disease (AD) model mice, including behavioral, histopathology and amyloid precursor protein (APP), protein kinase endoplasmic reticulum kinase (PERK) expressions. Method: The 42 4-month-old SPF-grade double transgenic (APP/PS1) mice were randomly divided into the high-dose group and the low-dose group, the donepezil hydrochloride group and the model group, and 10 C57BL mice of the same age were used as the blank group. Firstly, they were adapted to the environment for one week. After 2 months of treatment with different drug interventions, Morris water maze behavior was used to test the learning and memory abilities of each group of mice. After 1 month of treatment, histopathological changes in the hippocampus of each group of mice were observed by light microscopy. The expressions of APP, PERK protein and mRNA in the endoplasmic reticulum of hippocampus were detected by immunohistochemistry (IHC) and real-time fluorescent quantitative polymerase chain reaction (Real-time PCR). Result: After drug intervention, compared with the blank group, the escape latency of the AD model group was significantly prolonged (P<0.01), the neurons in the hippocampus of the model group were severely damaged, and the expressions of APP and PERK in the model group were significantly increased (P<0.01). In the comparison model group, the escape latency of the mice in each treatment group was significantly shortened (P<0.05,P<0.01), the degree of neuronal damage in the hippocampus of each treatment group was alleviated, and the positive expressions of APP and PERK in each treatment group were weakened (P<0.05). Conclusion: Hei Xiaoyaosan can significantly improve the learning and memory abilities of AD mice, which may be related to the reduction of the excessive stress response of endoplasmic reticulum to alleviate cognitive impairment in AD mice.
