Content Determination of Nine Constituents in Different Medicinal Parts of Pimpinella thellungiana
10.13422/j.cnki.syfjx.20191918
- VernacularTitle: 羊红膻9种成分的含量测定及不同药用部位比较
- Author:
Xiao-min CUI
1
;
Hui-li SHI
2
;
Hui REN
1
;
Jing HU
1
;
Mai-xia MENG
1
;
Juan CHEN
1
;
Xue MENG
1
;
Zhi-yong CHEN
1
Author Information
1. Shaanxi Academy of Traditional Chinese Medicine(TCM), Xi'an 710061, China
2. Shaanxi TCM Hospital, Xi'an 710003, China
- Publication Type:Research Article
- Keywords:
Pimpinella thellungiana;
different medicinal parts;
protocatechuic acid;
gallic acid;
neochlorogenic acid
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(24):97-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method to quantify nine constituents in different medicinal parts of Pimpinella thellungiana, in order to compare the content difference of the nine constituents, namely protocatechuic acid,gallic acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,luteolin-7-O-β-D-glucuronide,isochlorogenic acid A,apigenin-7-O-β-D-glucuronide and isochlorogenic acid C. Method:The analysis was performed on an Agilent TC-C18 column (4.6 mm×250 mm,5 μm) with acetonitrile and mixed acid solution (0.1% phosphoric acid-0.1% glacial acetic acid) as mobile phase for gradient elution. The handover detection wavelengths were at 265 and 325 nm. The column temperature was 20℃, and the flow rate was 1.0 mL·min-1. The experiment data was analyzed using the software of Markerlynx XS. Result:The nine constituents of protocatechuic acid,gallic acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid, luteolin-7-O-β-D-glucuronide,isochlorogenic acid A,apigenin-7-O-β-D-glucuronide and isochlorogenic acid C had a good degree of separation and a good linearity in their respective linear ranges(r>0.999 8). The average recoveries ranged from 99.11% to 100.76%,and the RSD ranged from 0.9% to 2.0% 。The results showed that the contents of the nine constituents had significant differences in different medicinal parts of P. thellungiana. The average contents of the nine constituents were the highest in leaves,which was followed by stem,and the lowest was in root. Conclusion:The study could provide evidence for the quality control,clinical application,and scientific resources utilization of P. thellungiana.
