Quantitative Determination of Four Active Constituents in Anti-inflammatory and Analgesic Active Fraction of Gaultheria leucocarpa var. yunnanensis
10.13422/j.cnki.syfjx.20192111
- VernacularTitle: HPLC测定民族药滇白珠抗炎镇痛活性部位(ARF)中4种活性成分的含量
- Author:
Xiao LI
1
;
Xiu-huan WANG
1
;
Yi-cheng ZHAO
1
;
Le WANG
1
;
Shu-sheng FAN
1
;
Xiao-ping WANG
1
;
Xiao XU
1
;
Xin YAN
1
;
Ting HE
1
;
Yan-li CHANG
1
;
Gai-mei SHE
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
- Publication Type:Research Article
- Keywords:
Gaultheria leucocarpa var. yunnanensis;
active fraction;
MSTG-A;
MSTG-B;
gaultherin;
chlorogenic acid;
quantitative determination
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(24):91-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a high performance liquid chromatography (HPLC) method for determination of four active constituents, namely MSTG-A,MSTG-B,gaultherin and chlorogenic acid in the anti-inflammatory and analgesic active fraction (ARF) of the ethnic medicine Gaultheria leucocarpa var. yunnanensis, in order to provide a methodological basis for the in-depth study and quality control of G. leucocarpa var. yunnanensis,and lay a foundation for later preparation and clinical application. Method:The determination was performed on COSMOSIL 5C18-PAQ (4.6 mm×250 mm,5 μm) column with methanol-0.2% glacial acetic acid (gradient elution) as the mobile phase at a flow rate of 1 mL·min-1. The column temperature was 25℃. The detection wavelength was set at 294 nm. Result:The linear range of MSTG-B,MSTG-A,gaultherin and chlorogenic acid were 0.014 06-0.450 00,0.007 81-0.250 00,0.003 13-0.100 00,0.000 94-0.030 00 g·L-1 (r ≥ 0.999 7),respectively,with a good precision,repeatability and stability. And the average recoveries were 100.81%,98.99%,96.12% and 102.56%,respectively. RSDs were 1.4%,0.7%,0.7%,2.4%,respectively. The contents of MSTG-B,MSTG-A,gaultherin and chlorogenic acid in ARF fraction of G. leucocarpa var. yunnanensis were 23.608,41.973,8.282,2.673 mg·g-1,respectively. Conclusion:The established method was simple and accurate, with a high repeatability. It can be used for determination of four active constituents in ARF fraction of G. leucocarpa var. yunnanensis,so as to provide a reference for the in-depth research,quality control and comprehensive evaluation of G. leucocarpa var. yunnanensis and lay a solid foundation for preparation and clinical application.
