Kinetic Analysis of Enzymatic Reaction of Active Components from Xinshao Formula in Liver Microsomes of Sham-Operated and Cerebral Ischemia-reperfusion Injury Rats
10.13422/j.cnki.syfjx.20182305
- VernacularTitle: 辛芍组方效应成分在正常和脑缺血再灌注损伤大鼠肝微粒体中的酶促反应动力学分析
- Author:
Zhi-bao LIU
1
;
Dan WU
2
;
Juan TANG
2
;
Hong SU
3
;
Yue-ting LI
2
;
Zi-peng GONG
2
;
Yong HUANG
2
;
Yong-lin WANG
2
Author Information
1. Daqing Oilfield General Hospital, Daqing 163312, China
2. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, School of Pharmacy, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550004, China
3. Daqing Medical College, Daqing 163312, China
- Publication Type:Research Article
- Keywords:
Xinshao fomula;
cytochrome P450;
enzymatic kinetics;
ischemia-reperfusion injury;
middle cerebral artery occlusion;
puerarin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):169-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate and compare enzymatic kinetics of scutellarin,apigenin-7-O-glucronide and paeoniflorin from Xinshao fomula in liver microsomes of sham-operated rats and middle cerebral artery occlusion(MCAO) rats with focal cerebral ischemia-reperfusion injury. Method: Xinshao fomula were incubated respectively with liver microsomes of sham-operated rats and MCAO rats,UPLC-MS and substrate elimination method was employed,Michaelis constant(Km),maximum velocity of enzymatic reaction(Vmax) and intrinsic clearance(CLint) of these three components from Xinshao fomula in liver microsomes of sham-operated rats and MCAO rats were calculated,these parameters between different groups were evaluated by statistical analysis. Result: The Km values of scutellarin,apigenin-7-O-glucronide and paeoniflorin in liver microsomes of MCAO rats were (0.798±0.031),(0.213±0.017),(0.499±0.029) μmol·L-1,which were quite different to these in liver microsomes of sham-operated rats.Compared with the sham-operated group,Vmax and CLint values of scutellarin and paeoniflorin in liver microsomes of MCAO rats were significantly reduced(P<0.05,P<0.01),and Vmax of apigenin-7-O-glucronide in liver microsomes of MCAO rat was also significantly reduced(P<0.05). Conclusion: Metabolic rates of these three active components from Xinshao fomula in liver microsomes of MCAO rats with focal cerebral ischemia-reperfusion injury decrease with low elimination rate.
