Effect of Canna edulis Type 3 Resistant Starch in Reducing Body Weight, Serum Lipid and Acute Toxicity
10.13422/j.cnki.syfjx.20190130
- VernacularTitle: RS3型芭蕉芋抗性淀粉的减肥降脂作用及急性毒性分析
- Author:
Min-yi QIU
1
;
Xiao-yan JIN
1
;
Cai-juan ZHANG
1
;
Chi ZHANG
1
;
Min WANG
1
;
Yan-bing LU
1
;
Bao-sheng ZHAO
1
;
Yuan ZHANG
2
;
Xue-yong WANG
1
Author Information
1. School of Chinese Materia Medica, Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2. College of Biochemical Engineering, Beijing Union University, Beijing 100023, China
- Publication Type:Research Article
- Keywords:
Canna edulis type 3 resistant starch;
obesity;
hyperlipidemia;
hypolipiclemtc effect;
acute toxicity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):149-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate effect of Canna edulis type 3 resistant starch(RS3) on weight loss and lipid reduction in obese hyperlipidemia mice and acute toxicity in mice. Method: KKAy mice were fed with high-fat diet for 20 weeks to establish a hyperlipidemia model and then randomly divided into model group,positive group (4 mg·kg-1), high-dose resistant starch group and low-dose resistant starch group (2,1 g·kg-1).Mice in normal group were fed with standard diet. The medication groups received corresponding drugs by gavage. Normal group and high-fat model group were given equal volume of deionized water. After 8 weeks,mice were put to death. The levels of total cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C),aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum of mice were measured,and weigh fat mass,fat/body ratio,body fat rate and Lee's index were calculated accurately. The pathological changes of liver and adipose tissue were observed byhematoxylin-eosin (HE). The acute toxicity of RS3 to mice was evaluated by limit test. The mice were continuously observed for 14 days, and the toxicity of mice was recorded. Result: The indicators of high-dose RS3 group were significantly reduced,such as body weight,fat mass,body fat rate,fat/body ratio,Lee's index,and serum TC,TG,LDL-C,AST,ALT levels(P<0.05).Histomorphometric examination showed that the administration of RS3 starch could significantly improve the fatty lesions of liver tissue, and the liver-protecting effect was obvious, which could still inhibit the expansion of fat cells and reduce the accumulation of fat in mice.Among them, the high-dose group wsa better;After the maximum dose of 36 g·kg-1 was administered,no toxic reaction and death occurred in the animals. Conclusion: RS3-type Canna Edulis Resistant Starch has a good effect in reducing body weight and serum lipid,with a better effect in the high-dose group and no toxicity. And the commonly used clinical dose is safe and reliable.
