Effect and Mechanism of Mori Folium on Insulin Resistance in 3T3-L1 Cells
10.13422/j.cnki.syfjx.20190128
- VernacularTitle: 桑叶改善3T3-L1细胞胰岛素抵抗的作用及其机制分析
- Author:
Min WANG
1
;
Ya-qi LI
1
;
Quan-tao MA
1
;
Chen-yue LIU
1
;
Chi ZHANG
1
;
Min-yi QIU
1
;
Cai-juan ZHANG
1
;
Ting WANG
1
;
Bao-sheng ZHAO
1
Author Information
1. School of Chinese Materia Medica, Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
Mori Folium;
serum containing drug;
type 2 diabetes mellitus (T2DM);
tumor necrosis factor-α(TNF-α);
insulin signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):135-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe effect of Mori Folium-containing serum on glucose consumption and cell activity of fat cell line 3T3-L1 insulin resistance (IR) model, in order to screen out the optimal concentration of drug-containing serum, detect effect of Mori Folium on the content of inflammatory factors, and explore the possible mechanism. Method: 3T3-L1 preadipocytes in logarithmic growth phase were selected, and induced with 10 mg·L-1 insulin (Ins), 0.25 mmol·L-1 dexamethasone (DEX) and 0.5 mmol·L-1 3-isobutyl-methylxanthine(IBMX) for 48 h and then with 10 mg·L-1 Ins for 48 h. After the cells were differentiated into mature adipocytes, they were induced with 1 μmol·L-1 DEX for 96 h to establish IR model. Glucose content in the supernatant of cells was detected by glucose oxidase after serum containing Mori Folium cultured for 12,24,36, 72 h. Methyl-thiazdyl-tetrazolium(MTT) was used to detect the effect of serum containing Mori Folium on IR cells activity. The content of tumor necrosis factor-α(TNF-α) was determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile, the effects of inflammatory factors on the expressions of insulin signaling pathway proteins insulin receptor (InsR), insulin receptor substrate (IRS), p-IRS1 and glucose transporter 4 (GLUT4) were determined by Western blot. Result: Serum containing Mori Folium could significantly increase the glucose consumption rate and cell activity of IR cells (P<0.01), reduce the content of TNF-α (P<0.01), and regulate the expression of insulin signaling pathway proteins (P<0.05,P<0.01). Conclusion: Mori Folium can significantly improve IR status of 3T3-L1 cells, and its mechanism may be related to inhibiting TNF-α and promoting the expressions of insulin signaling pathway proteins.
