Effects of oxygen glucose deprivation and reperfusion on gap junction and glucose uptake of astrocytes
10.3760/cma.j.issn.1674-6554.2019.11.002
- VernacularTitle: 氧糖剥夺再灌注对星形胶质细胞缝隙连接与糖摄取功能的影响
- Author:
Xiangyang ZHANG
1
;
Cuifang WANG
2
;
Weichun GUO
1
;
Yan CHEN
2
Author Information
1. Department of orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China
2. Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, China
- Publication Type:Journal Article
- Keywords:
Astrocyte;
Connexin 43;
Gap junction;
Oxygen-glucose deprivation;
Glucose uptake
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2019;28(11):967-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between gap junction and glucose uptake of astrocytes under oxygen-glucose deprivation(OGD) and reperfusion.
Methods:Cerebral cortical astrocyte from 1 day newborn SD rats were undergone the primary culture. The ischemia cell model was established by OGD. This experiment were divided into control group, OGD group and OGD+ CBX group.After different reperfusion time (0 h, 12 h 24 h and 48 h), the glucose uptake of astrocyte was measured by 2-NBDG through flow cytometry analysis and connexin 43(Cx43) gap junction plaques was detected using immunofluorescene.
Results:Compared with the control group, the glucose uptake of astrocyte was up-regulated induced by OGD following different reperfusion time.The glucose uptake of OGD group was (2.32±0.43)nmol/μgDNA in 24 hours reperfusion time and was (0.95±0.28)nmol/μgDNA in control group. The up-regulation was up to 2.63-fold increase (t=13.99, P=0.0024) in 24 hours after reperfusion.Compared with the control group, the Cx43 gap junction number was up to 2.5- fold increase(t=11.34, P=0.003) and the size was 1.85-fold increase (t=10.27, P=0.004) in 24 h reperfusion. The glucose uptake of astrocyte after OGD was reduced by CBX and the decrease was 42% in 48 h after reperfusion.
Conclusion:Those results urges us consider the clinical treatment for interfering with Cx43 gap junction.
