Appilcation of polycaprolactone/collagen nanofibrous membranes in constituting tissue engineering retinal pigment epithelium
10.3760/cma.j.issn.2095-0160.2019.11.007
- VernacularTitle: 聚己内酯/明胶纳米纤维膜在组织工程化视网膜色素上皮构建中的应用
- Author:
Tian TANG
1
;
Jie CHEN
1
;
Hua DENG
1
;
Xiaoguang YIN
2
;
Yan FU
1
Author Information
1. Second Outpatient Department, The General Hospital of Western Theater Command, Chengdu 610083, China
2. Chongqing Sidemu Biological Technology Company Limited, Chongqing 400026, China
- Publication Type:Journal Article
- Keywords:
Retinal pigment epithelium;
Retinal degeneration diseases;
Polycaprolactone;
Collagen;
Tissue engineering
- From:
Chinese Journal of Experimental Ophthalmology
2019;37(11):884-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential of polycaprolactone (PCL)/gelatin nanofibrous composite membrane in constituting tissue engineering retinal pigment epithelium (RPE).
Methods:The embryonic stem cells (ESCs) were differentiated into RPE (ESC-RPE) cells by spontaneous differentiation methods.Flow cytometry was used to detect the expression of specific RPE markers in ESC-RPE cells.The nanofiber membrane of PCL and PCL/gelatin nanofibrous composite membrane were prepared by electrospinning.The topography and contact angle of the two membranes were detected.The viability of ESC-RPE cells treated by the extracts of the two kinds of membranes was detected by cell counting kit-8 (CCK-8)method.After the ESC-RPE cells were cultured on the PCL/gelatin nanofibrous composite membrane, immunostaining was used to detect the expression of specific RPE markers of tissue engineering RPE.
Results:ESC-RPE cells were negative for pluripotent marker OCT4, but positive for microphthalmia-associated transcription factor (MITF), RPE65 and Bestrophin.Both the nanofiber membrane of PCL and PCL/gelatin nanofibrous composite membrane were porous and the contact angle were (97.2±3.1)° and (13.6±2.4)°, respectively.The extracts of the two kinds of membranes showed no significant effect on the growth of ESC-RPE cells.The tissue engineering RPE based on PCL/gelatin nanofibrous composite membrane positively expressed MITF and ZO-1.
Conclusions:PCL/gelatin nanofibrous composite membrane shows good hydrophilicity and biocompatibility.Besides, the membrane is favor for the growth of ESC-RPE cells and holds the potential to constitute tissue engineering RPE.
