Effects of Mongolian medicine Naru-3 on apoptosis of spinal cord injury model rats
10.3760/cma.j.issn.1673-4246.2019.12.014
- VernacularTitle: 蒙药那如三味丸对脊髓损伤模型大鼠细胞凋亡的影响
- Author:
Linbaiyila BU
1
;
Guisong HE
1
;
Bing HE
2
Author Information
1. The Orthopaedic of Panyu, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 511477, China
2. Institute of Chinese Medicine of Guangdong Pharmaceutical University, Guangzhou 510006, China
- Publication Type:Journal Article
- Keywords:
Spinal cord injury;
Apoptosis;
Mongolian drugs;
Naru-3;
Caspase 3;
Rats
- From:
International Journal of Traditional Chinese Medicine
2019;41(12):1343-1346
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of the Mongolian medicine on the apoptosis after spinal cord injury.
Methods:A total of 12 rats were divided into the sham group, the model group, and the like-3 treatment groups (Naru-3 group), 4 in each group. A rat model of spinal cord injury (SCI) was established by modified Allen's method. After 72 h, we sacrificed the rats, separated spinal cord cells, which were filled with 5×105 shop in every hole, training for subsequent testing after 24 h. The relative expression of caspase-3 was detected by RT-QPCR and western blot, and apoptosis was analyzed by flow cytometry.
Results:Compared to the model group, the expression of caspase-3 mRNA (2.177 ± 0.084 vs. 3.287 ± 0.098) and caspase-3 protine (1.063 ± 0.157 vs. 2.180 ± 0.147) in the Naru-3 group significantly decreased (P<0.05). The apoptosis rate (11.400% ± 0.953% vs. 14.260% ± 1.105%) of the spinal cord in the Naru-3 group significantly decreased (P<0.05).
Conclusions:The Naru-3 solution can reduce the rate of spinal cord cell apoptosis after spinal cord injury, its mechanism may be related to the suppressed caspase-3 protein expression.
