Analysis on the transmission characteristics of newly reported human immunodeficiency virus/acquired immunodeficiency syndrome cases based on the molecular transmission network in Huzhou, Zhejiang, 2017

Wanjun CHEN; Xiaohong PAN; Qin FAN; Mingyu LUO; Jing LI; Jun JIANG; Zhongrong YANG; Jiafeng ZHANG

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Analysis on the transmission characteristics of newly reported human immunodeficiency virus/acquired immunodeficiency syndrome cases based on the molecular transmission network in Huzhou, Zhejiang, 2017

VernacularTitle: 应用分子传播网络分析浙江省湖州市2017年新报告HIV感染者和艾滋病患者的传播特征
Author: Wanjun CHEN ¹ ; Xiaohong PAN ¹ ; Qin FAN ¹ ; Mingyu LUO ¹ ; Jing LI ² ; Jun JIANG ¹ ; Zhongrong YANG ² ; Jiafeng ZHANG ¹
Author Information

1. Department of AIDS and STD Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhuo 310051, China
2. Division of AIDS and Tuberculosis Control and Prevention, Huzhou Center for Disease Control and Prevention, Huzhou 313000, China
Publication Type:Journal Article
Keywords: HIV; Molecular epidemiology; Molecular typing; Molecular transmission network; Transmission characteristics
From: Chinese Journal of Preventive Medicine 2019;53(12):1278-1283
CountryChina
Language:Chinese
Abstract: Objective:Using field epidemiological investigation and molecular analysis to construct the molecular transmission network of human immunodeficiency virus/acquired immunodeficiency syndrome cases (HIV/AIDS) newly diagnosed in Huzhou in 2017, Zhejiang Province.
Methods:A total of 160 participants were obtained through a web-based system from Chinese Center for Disease Control and Prevention (CCDC) with the features of diagnosed in Huzhou in 2017 who also had been collected samples for the first follow-up. The basic information of demographic characteristics and risk factors was extracted from the website. RNA was extracted from plasma samples of untreated cases, followed by RT-PCR and nest-PCR for pol gene amplification, sequencing. Phylogenetic tree was constructed by MEGA software for HIV gene subtyping. TN93 model was used for calculating the distance between two sequences. Cytoscape software was used for drawing molecular transmission network. And then an epidemiological survey was conducted to cases in the primary cluster.
Results:A total of 138 sequenced individuals (86.3%) were acquired from 160 individuals. Among which, 123 (89.1%) were male. The highest proportion of subtype was CRF07_BC (60, 43.5%), followed by CRF01_AE (46, 33.3%), and with four cases of Unique Recombinant Form (URF, CRF01_AE and CRF07_BC) and one case of URF (subtype B and C). A total of 18 molecular clusters included 56 individuals (40.6%) were found in the transmission network under the optimal genetic distance threshold (1.0%). The clustering proportion of CRF07_BC (66.1%, 37 cases) was higher than that of CRF01_AE. There were 9 clusters formed among CRF07_BC, including 37 cases (accounting for 61.7%, 37/60). The primary transmission cluster contained 11 cases, among which 9 cases were transmitted by homosexual sex. The first time of the cases to have homosexual behavior is range from 2010 to 2016, whose media number (P₂₅, P₇₅) of partners was 6 (3.5, 8.5). Most of the cases come from Anhui Province and engaged in garment industry (5 cases), between which there were 8 cases used Blued software to seek for casual partners, 1 case seeking for casual partners in garden.
Conclusion:With CRF07_BC and CRF01_AE predominantly circulating, HIV genetic diversity had been noticed in this area. The primary cluster was consisted of high proportion of locally new infections, and a specific population aggregation in limited place existed.