The parametric measurementof physico-chemical parameter and three types of effective components in Jiedu-Zhixue Decoction
10.3760/cma.j.issn.1673-4246.2020.01.013
- VernacularTitle: 解毒止血汤水提液中黄芩苷、汉黄芩苷、丹皮酚含量及其理化参数测定
- Author:
Limei LIU
1
;
Song XIE
1
;
Wei PENG
1
;
Meng XIANG
1
;
Hongyan GUO
1
;
He TU
1
Author Information
1. Department of Pharmaceutical Preparation, Sichuan Provincial Orthopedic Hospital, Chengdu 610041, China
- Publication Type:Journal Article
- Keywords:
Dosage forms improving;
Jiedu-Zhixue Decoction;
Baicaline;
Wogonoside;
Paeonol;
Physico-chemical parameter;
High performance liquid chromatography;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2020;42(1):62-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To set up a method to measure the content of baicaline, wogonoside, and paeonol in Jiedu-Zhixue Decoction extract and to measure their physico-chemical parameters.
Methods:By using HPLC analytical methods to measure the content of baicaline, wogonoside, and paeonol in Jiedu-Zhixue Decoction extract. The RP18 column was adopted, gradient eluted with mobile phase of acetonitrile-1% phosphoric acid water, flow rate was 1 ml/min, detection wavelength was 274 nm, and the column temperature was 30 ℃. The method quantitatively analyze the compound extracts of baicaline, wogonoside and paeonol were used.
Results:The method isrepeatable, stable with good recorey rates. The calibration curves of baicaline was in good linearity over the range of 0.191 2-1.147 2 μg. The calibration curves of wogonoside was in good linearity over the range of 0.041 5-0.207 4 μg. The calibration curves of paeonol was in good linearity over the range of 0.019 5-0.156 3 μg. There was no significant difference among the component of 3 different batches of Jiedu-Zhixue Decoction extract, and the pH value range was 5.240-5.753, relative density range was 0.846-0.889.
Conclusions:The method used in this stydy to measure the effective components of Jiedu-Zhixue Decoction extract was stable and feasible, which is suitable for quality control of Jiedu-Zhixue Decoction.
