Effect of Fengshi Qutong Capsule on Collagen-induced Arthritis of Rats
10.13422/j.cnki.syfjx.20190228
- VernacularTitle: 风湿祛痛胶囊对大鼠胶原诱导性关节炎的影响
- Author:
Jing-xia WANG
1
;
Chun-fang LIU
2
;
Lian-hua HE
2
;
Cong-cong SUN
2
;
Yi-qun LI
2
;
Hao-ye MENG
3
;
Jiang PENG
3
;
Yu JING
4
;
Yan-dong MIAO
4
;
Na LIN
1
Author Information
1. Chengde Medical University, Chengde 067000, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China
3. Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China
4. Tonghua Golden-Horse Group, Beijing 100028, China
- Publication Type:Research Article
- Keywords:
Fengshi Qutong capsule;
collagen-induced arthritis;
histopathology;
radiography;
cytokines
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):89-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the intervention effect of Fengshi Qutong capsule on collagen-induced arthritis (CIA) in rats. Method:SD rats were randomly divided into normal group, model group, low, medium and high-dose Fengshi Qutong capsule groups (0.25, 0.5, 1 g·kg-1·d-1), and methotrexate group(0.2 mg·kg-1·d-1).Except for normal group, the other groups were immunized with type Ⅱ collagen and incomplete Freund's adjuvant to establish a CIA model. On the 1st day after the first immunization, the administration group was given intragastric administration, once a day, for 19 days; on the 8th day after the first immunization, the symptoms of joint swelling and malformation of the rats were observed, and the clinical scores and incidence of arthritis were evaluated. On the 19th day, micro-computed tomography and bone metrology were performed, and histopathological examination of inflammatory joints was performed,andsynovial inflammation,vasospasm, cartilage erosion and bone destruction by pathological severity scores, serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF),matrix metalloproteinase-3 (MMP-3) and receptor activator of nuclear factor kappa B ligand(RANKL)were detected by enzyme linked immunosorbent assay. Result:Fengshi Qutong capsule could improve the symptoms of inflammatory joint redness, swelling and deformity in CIA rats in a dose-dependent manner. Compared with normal group, clinical score and incidence, joint synovial inflammation, vasospasm, cartilage erosion and pathological score of bone destruction in joint group were significantly increased (P<0.01), bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular thickness (Tb. Th), trabecularnumber (Tb. N) were significantly decreased (P<0.01), bone surface area/bone volume (BS/BV) and trabecular separation (Tb. Sp) were significantly elevated (P<0.01),and levels of IL-1β, TNF-α, VEGF, MMP-3 and RANKL in serum were increased (P<0.01); compared with model group, clinical scores and incidence, joint synovial inflammation, vasospasm, cartilage erosion and bone destruction of each drug groupwere significantly lower (P<0.05, P<0.01), BMD, BV/TV, Tb.Th, Tb.N were significantly elevated (P<0.05, P<0.01), BS/BV and Tb.Sp were significantly decreased (P<0.05, P<0.01), and levels of serum IL-1β, TNF-α, VEGF, MMP-3 and RANKL were significantly decreased (P<0.05, P<0.01). And the control drug methotrexate had similar effects to the high-dose group. Conclusion:Fengshi Qutong capsule can effectively alleviate the clinical symptoms and conditions of experimental rheumatoid arthritis in rats, reduce the incidence, and relieve the histopathology and imaging severity, while inhibiting the inflammatory cytokines.
