Analysis of factors influencing the mobilization and collection of autologous peripheral blood stem cells
10.3760/cma.j.issn.1009-9921.2019.09.005
- VernacularTitle: 自体外周血干细胞动员及采集效果影响因素分析
- Author:
Zhiqiang ZHAO
1
;
Lieyang WANG
1
;
Xiaolan LIU
2
;
Jiangtao WANG
2
;
Tao GUAN
2
;
Zong ZHANG
2
;
Yanhong LUO
3
;
Liping SU
1
Author Information
1. Department of Hematology, Shanxi Provincial Cancer Hospital, Taiyuan 030013, China
2. Department of Hematology Laboratory, Shanxi Provincial Cancer Hospital, Taiyuan 030013, China
3. Department of Health Statistics, Shanxi Medical University, Taiyuan 030001, China
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell mobilization;
Peripheral blood stem cell transplantation;
Transplantation, autologous
- From:
Journal of Leukemia & Lymphoma
2019;28(9):533-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the factors influencing the mobilization and collection of autologous peripheral blood stem cells.
Methods:The clinical data of 62 patients who received autologous peripheral blood hematopoietic stem cell mobilization in Shanxi Provincial Cancer Hospital from April 2012 to March 2017 were collected. The effects of age, gender, disease type, chemotherapy cycle, disease status, different schemes and the number of CD34+ cells in peripheral blood of patients 1 d before collection on the number of CD34+ cells and the success rate of CD34+ cells collection were analyzed. Measurement data were compared by one-way ANOVA and t test; count data were compared by χ 2 test; multivariate analysis was performed by multiple linear regression analysis.
Results:There were statistically significant differences in the number of CD34+ cells between patients with chemotherapy >6 cycles and ≤6 cycles [(2.6±1.3)×106/kg vs. (5.8±2.2)×106/kg; t = 5.221, P < 0.01], and the difference in the success rate of CD34+ cell collection between the two groups was statistically significant [68.8% (11/16) vs. 97.8% (45/46); χ2 = 8.396, P = 0.004]. The difference in the CD34+ cells yield was not statistical significance between male and female patients [(5.4±2.2)×106/kg vs. (4.5±2.8)×106/kg; t = 1.302, P = 0.198)], but the collection success rate in males was higher than that in females [97.6% (40/41) vs. 76.2% (16/21)], and the difference was statistically significant (χ 2 = 5.017, P = 0.025). The success rate of CD34+ cell collection in patients with ≥10/μl CD34+ cell in the peripheral blood was significantly higher than that in patients with < 10/μl CD34+ cells 1 d before the collection[97.9% (47/48) vs. 64.3% (9/14)], and the difference was statistically significant (χ 2 = 10.668, P = 0.001). The differences in CD34+ cells yield and collection success rate between patients with different age, disease type, disease status and mobilization regimen were not statistically significant (all P > 0.05). Multi-factor analysis showed that > 6 cycles chemotherapy before mobilization was the adverse factor affecting stem cell collection (b = -3.435, P < 0.01).
Conclusions:The effective mobilization and collection of autologous peripheral blood stem cells are related to the number of chemotherapy cycles before mobilization. The stem cell mobilization and collection should be conducted as soon as possible when the chemotherapy is ≤ 6 cycles and the patient reaches partial remission or above. In addition, peripheral blood CD34+ cell count should be monitored during mobilization. When the peripheral blood CD34+ cell count is > 10/μl, the collection could be started on the next day to obtain a better collection effect, so as to improve the success rate of collection.
