Observation of panobinostat alleviates early brain injury in subarachnoid hemorrhage model
10.3760/cma.j.issn.1008-6706.2019.09.017
- VernacularTitle: Panobinostat改善SD大鼠蛛网膜下腔出血后早期脑损伤的观察
- Author:
Jiaxing ZHANG
1
;
Yebin ZHANG
1
;
Liqiang WU
2
Author Information
1. Department of Neurosurgery, Shenzhen Hospital(GuangMing) Affliated to University of Chinese Academy of Sciences, Shenzhen, Guangdong 518106, China
2. Department of Neurosurgery, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, China
- Publication Type:Journal Article
- Keywords:
Subarachnoid hemorrhage;
Brain injury;
Panobinostat (LBH589);
Histone deacetylase(HDAC)/Enzyme Inhibitor;
Neurons;
Apoptosis;
Acetylation;
Animals
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(9):1092-1096
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of panobinostat (LBH589) on the early brain injury (EBI) in the model of subarachnoid hemorrhage(SAH) in SD rats.
Methods:SD rats were randomly divided into 4 groups: sham(10 rats) and SAH(10 rats), SAH+ vehicle(20 rats) and SAH+ Panobinostat(20 rats). Drug or vehicle was given by lateral-ventricular stereotaxic injection 24h before the SAH model was introduced.Water contents and the neurological scores were determined at 24h post-SAH.The levels of Ac-H3K27 in frontal and lateral lobe were detected by Western blot.
Results:The mean neurological score of the SAH group was higher than that of the sham group(F=13.000, P=0.007). The water content of the SAH group was higher than that of the sham group (F=8.229, P=0.019). The level of Ac-H3K27 was higher in the SAH+ Panobinostat group than that in the SAH+ vehicle group(F=41.250, P=0.000). The mean neurological score of the SAH+ Panobinostat group was lower than that of the SAH+ vehicle group(F=9.560, P=0.011). The water content of the SAH+ Panobinostat group was lower than that of the SAH+ vehicle group(F=8.211, P=0.020). The correlation analysis indicated that the level of acetylation of H3 was negatively correlated with the neurological score(r=-0.585, P=0.046).
Conclusion:Panobinostat can improve the neurological behavior and alleviate early brain injury in the SAH model.
