Effect of taurine on the antioxidant capacity and ATP activity of skeletal muscle in rats with exhaustive exercise
10.3760/cma.j.issn.1008-6706.2019.09.002
- VernacularTitle: 牛磺酸对力竭运动大鼠骨骼肌抗氧化能力和三磷酸腺苷活性的影响
- Author:
Peng ZHAO
1
;
Shuijun ZHANG
2
Author Information
1. Department of Orthopedics and Traumatology, the Traditional Chinese Medicine Hospital of Xiaoshan District, Hangzhou, Zhejiang 311201, China
2. Department of Orthopedics, the People's Hospital of Zhejiang Province, Hangzhou, Zhejiang 310014, China
- Publication Type:Journal Article
- Keywords:
Taurine;
Exercise test;
Muscle fibers, skeletal;
Oxidative coupling;
Adenosine triphosphate;
Rats
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(9):1029-1032
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of taurine on the activity of SOD, MDA, Na+ -K+ -ATP enzyme and Bax/Bcl-2 in skeletal muscle of rats after exhaustive exercise.
Methods:Thirty male SD rats(6 months old) were randomly divided into normal group, control group and taurine group, with 10 rats in each group.The normal group was given routine feeding for 1 week, adaptive training for 3 days, without applying other measures.The control group was given routine feeding for 1 week, after adaptive training for 3 days underwent exhaustive exercise for 2 days.The taurine group was given 200mg/kg taurine gavage daily on the basis of the control group.The activity of SOD, MDA, Na+ -K+ -ATP and Bax/Bcl-2 were measured in the skeletal muscle after 2 days exhaustive exercise.
Results:After 2 days exhaustive exercise, the SOD concentration in skeletal muscle of the control group was (146.58±13.42)U/mg prot, which was lower than that in the taurine group[(143.81±15.93)U/mg prot] (t=2.519, P<0.05). The MDA concentration in the skeletal muscle of the control group was (1.97±0.20)nmol/mg prot, which was higher than that in the taurine group [(1.22±0.19)nmol/mg prot] (t=2.356, P<0.05). The ratio of SOD/MDA in the control group was (60.86±20.38), which was lower than that in the taurine group [(120.87±23.51)] (t=4.071, P<0.05). The activity of Na+ -K+ -ATP in the control group was (2.42±0.67)U/mg prot, which was lower than that in the taurine group [(5.74±1.15)U/mg prot] (t=3.905, P<0.05). The ratio of Bax/Bcl-2 in the control group was (1.62±0.17), which was higher than that in the taurine group [(0.96±0.14)] (t=3.419, P<0.05).
Conclusion:Taking taurine before exhaustive exercise can increase the ability of scavenging free radicals, reduce the production of oxidative stress products and protect the activity of Na+ -K+ -ATP enzyme.It is benefit for maintain the stability of cell environment and prevent skeletal muscle injury.
