Value of serum HBV RNA in HBeAg-negative patients with chronic hepatitis B
10.3760/cma.j.issn.1007-3418.2019.09.002
- VernacularTitle: 血清HBV RNA在HBeAg阴性慢性乙型肝炎患者中的检测价值
- Author:
Bei JIANG
1
;
Chang LIU
;
Rui SU
;
Chao MENG
;
Yu CAO
;
Xiaoya ZHENG
;
Wenjuan REN
;
Feinan LYU
;
Wei LU
Author Information
1. Tianjin Second People’s Hospital, Tianjin institute of Hepatology, Tianjin 300192, China
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Hepatitis B e antigens;
HBV RNA
- From:
Chinese Journal of Hepatology
2019;27(9):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze serum HBV-RNA levels in patients with chronic hepatitis B whose serum HBV-DNA has dropped to undetected levels after treatment with entecavir, and to explore the correlation between HBV-RNA level and liver biochemical parameters, which lay the research foundation for the clinical significance of new serological marker HBV-RNA.
Methods:HBeAg negatively detected 107 cases with chronic hepatitis B whose serum HBV-DNA test results were lower than detection level for six consecutive months after receiving standard nucleoside therapy for more than 12 months were included. HBV-RNA level was detected by Perkin-Elmer reagent. HBV-DNA level was detected by Roche Cobas. Hitachi automatic biochemical analyzer was used to detect ALT and AST. Architect chemiluminescence analyzer was used to detect HBsAg, HBeAg, anti-HBe and anti-HBc. RStudio software was performed to analyze the correlation between HBV-RNA level and liver biochemical parameters. Logistic regression was used to analyze the independent factors influencing HBV-RNA level.
Results:The positive detection rate of serum HBV-RNA in patients with chronic hepatitis B whose serum HBV-DNA had dropped to undetected levels after ETV treatment was 22.43%. HBsAg, ALT and AST levels in HBV-RNA positive group were slightly higher than HBV-RNA negative group, while anti-HBc levels were slightly higher in HBV-RNA negative group. There was no difference in the level of anti-HBe between the HBV-RNA negative and the positive group. Logistic regression analysis showed that anti-HBc was an independent factor influencing the level of HBV-RNA detection (P = 0.021).
Conclusion:HBV-RNA can be detected in some patients with chronic hepatitis B whose serum HBV-DNA level has dropped to undetected levels after ETV treatment. Serum HBV-RNA only comes from the direct transcription of cccDNA, so it is better than HBV-DNA and HBsAg to reflect cccDNA level or activity. Anti-HBc, as an independent factor influencing the level of HBV-RNA, may be used in combination as a new marker to predict the efficacy of antiviral therapy.
