Establishment of pancreatic cancer organoids with endoscopic ultrasonography-guided fine-needle aspiration specimen: a prospective pilot study
10.3760/cma.j.issn.1007-5232.2019.09.008
- VernacularTitle: 内镜超声引导下细针抽吸术获取胰腺癌组织构建体外三维类器官模型的前瞻性临床研究
- Author:
Jie YANG
1
;
Shu ZHANG
2
;
Yan ZHU
2
;
Guifang XU
2
;
Song ZHANG
2
;
Chunyan PENG
2
;
Jun YANG
3
;
Lei WANG
2
;
Xiaoping ZOU
2
;
Ying LYU
4
Author Information
1. Drum Tower Clinical College of Nanjing Medical University, Nanjing 210008, China
2. Department of Gastroenterology, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing 210008, China
3. Department of Pathology, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing 210008, China
4. Drum Tower Clinical College of Nanjing Medical University, Nanjing 210008, China; Department of Gastroenterology, Nanjing Drum Tower Hospital Affiliated to Nanjing University Medical School, Nanjing 210008, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, pancreatic ductal;
Endoscopic ultrasonography-guided fine-needle aspiration;
Organoid;
Precision medicine
- From:
Chinese Journal of Digestive Endoscopy
2019;36(9):649-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the feasibility of establishing human 3D pancreatic cancer organoids with endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) specimen.
Methods:A total of 9 patients with suspected pancreatic masses were prospectively included in this study from June 2017 to January 2018. EUS-FNA was performed for initial diagnosis. The biopsy tissues were obtained from a COOK 22-gauge FNA needle for organoid establishment, and the growth status in vitro was observed.
Results:Nine specimens of pancreatic lesions obtained from EUS-FNA were enrolled. Successful establishment of organoids was achieved in 5 cases, which were all confirmed to be pancreatic cancer histopathologically. In the process of generating, the growth rate of organoids increased correspondingly. The pathological morphology of these organoids was similar to the corresponding pancreatic tissues in HE staining.
Conclusion:Pancreatic cancer organoids can be successfully created by means of EUS-FNA. Establishment of these organoids can potentially provide excellent models for patients with pancreatic cancer in guiding precision treatment.
