Effect of hydrogen on mitochondrial function in brain tissues of mice with sepsis-associated encephalopathy
10.3760/cma.j.issn.0254-1416.2019.10.020
- VernacularTitle: 氢对脓毒症相关性脑病小鼠脑组织线粒体功能的影响
- Author:
Keliang XIE
1
;
Yaoqi WANG
1
;
Yi JIANG
1
;
Xing MAO
1
;
Hongguang CHEN
1
;
Yang ZHANG
2
;
Yonghao YU
1
Author Information
1. Department of Anesthesiology, General Hospital of Tianjin Medical University Tianjin Institute of Anesthesiology, Tianjin 300052, China
2. Department of Anesthesiology, Tianjin Fourth Center Hospital, Tianjin 300140, China
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Sepsis;
Brain;
Mitochondria
- From:
Chinese Journal of Anesthesiology
2019;39(10):1228-1232
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of hydrogen on the mitochondrial function in brain tissues of mice with sepsis-associated encephalopathy (SAE).
Methods:Two hundred and sixty-eight healthy male C57 mice, aged 6 weeks, weighing 20-25 g, were divided into 4 groups (n=67 each) using a random number table method: sham operation group (group SH), sham operation plus hydrogen gas group (group SH+ H2), group SAE, and SAE plus hydrogen gas group (group SAE+ H2). Sepsis was produced by cecal ligation and puncture in anesthetized mice.The mice in group SH+ H2 and group SAE+ H2 inhaled 2% hydrogen gas for 1 h starting from 1 and 6 h after operation, respectively.The postoperative 7-day survival rate was recorded.Brain tissues were obtained at 24 h after operation to count the normal neurons in hippocampal CA1 region.At 6, 12 and 24 h after operation, hippocampal mitochondria were isolated for determination of mitochondrial membrane potential (MMP) by fluorescence spectrophotometry and ATP content by a bioluminescence assay.Y-maze (spontaneous alternation) test was performed at days 3, 5 and 7 after operation.
Results:Compared with group SH, the 7-day survival rate was significantly decreased, the number of normal neurons in hippocampal CA1 region, MMP, mitochondrial ATP content and spontaneous alternation percentage in Y-maze test were significantly decreased in group SAE and group SAE+ H2 (P<0.05). Compared with group SAE, the 7-day survival rate, the number of normal neurons in hippocampal CA1 region, MMP, mitochondrial ATP content and spontaneous alternation percentage in Y-maze test were significantly increased in group SAE+ H2 (P<0.05).
Conclusion:The mechanism by which hydrogen reduces SAE is probably associated with improving mitochondrial function in brain tissues of mice.
