The alteration of lncRNA expression in peripheral blood mononuclear cells and the effects of anti-anxiety treatment on lncRNA expression of generalized anxiety disorder patients
10.3760/cma.j.issn.1674-6554.2019.10.002
- VernacularTitle: 广泛性焦虑症外周血单核细胞长链非编码RNA异常表达及抗焦虑治疗对其表达水平的影响
- Author:
Lingming KONG
1
;
Xiaoli ZHU
1
;
Wei NIU
1
;
Mingjun HE
1
;
Gaofeng YAO
1
;
Shengdong CHEN
2
;
Liyi ZHANG
1
Author Information
1. Mental Disorder Prevention & Treatment Institution, No.904 Hospital of Joint Logistics Support Force, Changzhou 213003, China
2. Neurological Department, No.904 Hospital of Joint Logistics Support Force, Changzhou 213003, China
- Publication Type:Clinical Trail
- Keywords:
Generalized anxiety disorder;
Peripheral blood mononuclear cell;
lncRNA;
Differential expression
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2019;28(10):870-874
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To confirm expression alteration of long non-coding RNA(lncRNA) in peripheral blood mononuclear cells (PBMCs) of generalized anxiety disorder(GAD) patients and anti-anxiety treatment effects on aberrant expression of lncRNAs.
Methods:Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was employed in 80 GAD patients and 40 healthy participants to confirm 10 aberrant lncRNAs screened by microarray expression profiling.And 26 out of all the 80 GAD patients were recruited for lncRNA expression level testing and Hamilton Anxiety Scale(HAMA) assessments before and after 6 weeks’ treatment.
Results:Six of ten lncRNAs selected by array profiling (lncRNA4(7.44±2.26), lncRNA5(6.83±2.28), lncRNA6(8.09±2.30), lncRNA8(9.10±2.36), lncRNA9(7.66±2.12), lncRNA10(7.34±2.12)) were verified by qRT-PCR that the lncRNA expression levels were significantly up regulated in GAD patients compared with healthy controls(Z=-3.022--1.996, P<0.05 or 0.01), and lncRNA4(9.73±2.53), lncRNA6(9.91±2.01), lncRNA8(10.48±1.68), lncRNA9(9.02±1.58), lncRNA10(9.04±2.08) were down regulated significantly after 6 weeks’ anti-anxiety treatment(Z=-3.180--2.530, P<0.05 or 0.01) along with signicant reduction of total HAMA score (11.19±8.37), dimension scores of somatic anxiety(5.31±4.76), psychic anxiety(5.88±3.82) (t=5.502-5.971, P<0.01). The alterations of lncRNA4, lncRNA6, lncRNA8, lncRNA9, lncRNA10 were positively correlated with that of HAMA total score and psychic anxiety score(r=0.39-0.69, P<0.05 or 0.01), and alteration of lncRNA6, lncRNA8, lncRNA10 had positive correlation with that of somatic anxiety score(r=0.44-0.59, P<0.01).
Conclusion:The expression level of lncRNA4, lncRNA5, lncRNA6, lncRNA8, lncRNA9, lncRNA10 are up-regulation in PBMCs of GAD patients and anti-anxiety treatment can reverse the expression level of lncRNAs. Alteration of lncRNA expression has osculatory association with improvement of anxious symptom.
