Evidence for the Presence of a cis-acting Element in the Coding Region of the Japanese Encephalitis Virus Capsid Protein.
10.4167/jbv.2006.36.2.125
- Author:
Sang Im YUN
1
;
Yu Jeong CHOI
;
Byung Hak SONG
;
Jeong Min KIM
;
Shien Young KANG
;
Young Min LEE
Author Information
1. Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea. ymlee@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Japanese encephalitis virus;
cis-acting element;
Capsid protein;
Viral replication
- MeSH:
Asian Continental Ancestry Group*;
Capsid Proteins;
Clinical Coding*;
Cyclization;
Encephalitis Virus, Japanese*;
Encephalitis, Japanese*;
Flavivirus;
Humans;
Replicon;
RNA
- From:Journal of Bacteriology and Virology
2006;36(2):125-132
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Japanese encephalitis virus (JEV) is a member of mosquito-borne flaviviruses. To investigate whether there is a cis-acting genetic element in the coding region of the JEV C protein, which is required for viral replication, we generated four mutants by introducing a various size of deletions in each structural protein-coding region, designated as pJEV/Rep/deltaCC/LUC, pJEV/Rep/deltaC/LUC, pJEV/Rep/deltaprM/LUC, and pJEV/Rep/deltaE/LUC, of these, all replicons except for pJEV/Rep/deltaCC/LUC were competent in replication. Since pJEV/Rep/deltaCC/LUC is the same as pJEV/Rep/ deltaC/LUC except for an additional 5' deletion (nt 132~201) in the coding region of the C protein, this region appeared to be essential for RNA replication. This is consistent with the proposed cyclization sequence motif in the 5' region of the C gene, which has been recently shown to be required for replication in other mosquito-borne flaviviruses such as DV, YFV, KUN, and WNV. Thus, our results suggest that a cis-acting genetic element in the coding region of the JEV C protein may play an important role in RNA replication. This study will facilitate the current understanding of JEV RNA replication.
