Association of Killer Cell Ig-like Receptor (KIR) with an Adaptor Protein Shc.
- Author:
Hyun Il CHO
1
;
Yong Joon CHWAE
;
Sang Myun PARK
;
Jongsun KIM
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: KIR; Shc; SHP-1; SHP-2; cell activation; cell proliferation; inhibitory signal transduction
- MeSH: Cell Proliferation; Cytoplasm; Killer Cells, Natural; Phosphotyrosine; Protein Tyrosine Phosphatases; T-Lymphocytes; Transfection
- From:Immune Network 2006;6(2):67-75
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Cytotoxic function of killer cells is inhibited by specific recognition of class I MHC molecules on target cells by inhibitory killer Ig-like receptors (KIR) expressed on NK cells and some cytotoxic T cells. The inhibitory effect of KIR is accomplished by recruitment of SH2-containing protein tyrosine phosphatase (SHP) to the phosphotyrosine residues in the cytoplasmic tail. METHODS: By in vitro coprecipitation experiments and transfection analysis, we investigated the association of KIR with an adaptor protein Shc in Jurkat T cells. RESULTS: The cytoplasmic tail of KIR appeared to associate with an adaptor protein Shc in Jurkat T cell lysates. Similar in vitro experiments showed that phosphorylated KIR cytoplasmic tail bound SHP-1 and Shc in Jurkat T cell lysates. The association of KIR with Shc was further confirmed by transfection analysis in 293T cells. Interestingly, however, Shc appeared to be replaced by SHP-2 upon engagement of KIR in 293T cells. CONCLUSION: Our data indicate that KIR associate with an adaptor protein Shc in Jurkat T cells, and suggest that KIR might have an additional role which is mediated by this adaptor protein.
