Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice.
10.4142/jvs.2010.11.3.213
- Author:
Bock Gie JUNG
1
;
Sun Ju CHO
;
Jae Hyung KO
;
Bong Joo LEE
Author Information
1. College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. bjlee@chonnam.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
atopic dermatitis;
IL-10;
NC/Nga mice;
plasmid DNA
- MeSH:
Animals;
Case-Control Studies;
DNA Primers/genetics;
Dermatitis, Atopic/immunology/*prevention & control;
Disease Models, Animal;
Dogs;
Female;
Interleukin-10/genetics/*immunology/*therapeutic use;
Mice;
Mice, Mutant Strains;
Plasmids/genetics/*therapeutic use;
Staphylococcus aureus/isolation & purification;
Statistics, Nonparametric;
T-Lymphocytes, Helper-Inducer/*immunology
- From:Journal of Veterinary Science
2010;11(3):213-220
- CountryRepublic of Korea
- Language:English
-
Abstract:
Interleukin (IL)-10 exerts potent anti-inflammatory effects by suppression of both T-help (Th) 1 and Th2 cells. Previous studies have reported that IL-10 can ameliorate various inflammatory disorders. The present study was performed to examine whether IL-10 plasmid DNA could suppress development of atopic dermatitis (AD)-like skin lesions in NC/Nga mice, as an initial step towards the development of an appliance for use in dogs with AD. Intradermal injection of IL-10 plasmid DNA markedly inhibited the development of AD-like skin lesions, as evidenced by a marked decrease in skin symptoms and reduced inflammation within the skin lesions. Efficacy was confirmed by significant decreases in eosinophil ratio and serum IgE concentration, and a reduction in the number of Staphylococcus aureus recovered from the ear. Moreover, relative mRNA expression levels of IL-4 and interferon-gamma in the skin lesions of mice injected with IL-10 plasmid DNA were also decreased compared with those of control mice. Of note, higher serum IL-10 levels in mice injected with IL-10 plasmid DNA were maintained compared with those in control mice. Taken together, the results indicate that IL-10 plasmid DNA can suppress the development of AD-like skin lesions by suppressing both Th1 and Th2 cell responses. Beneficial effects of IL-10 plasmid DNA may be expected in dogs with AD.
