miR-1297 promotes malignant biological behaviors of breast cancer MCF-7 cells by down-regulating TET3
10.3872/j.issn.1007-385x.2019.10.014
- VernacularTitle:miR-1297通过下调TET3促进乳腺癌MCF-7细胞的恶性生物学行为
- Author:
ZHAO Xueyun
1
;
LI Yuanping
1
;
ZHANG Yingyi
1
;
ZHU Qin
1
;
HUANG Liang
1
;
ZHANG Qiang
1
Author Information
1. (Department of Thyroid and Breast Surgery, People’s Hospital of Leshan City,
- Publication Type:Journal Article
- Keywords:
breast cancer;
MCF-7 cell;
miR-1297;
TET3;
epithelial-mesenchymal transition (EMT);
proliferation;
invasion;
migration
- From:
Chinese Journal of Cancer Biotherapy
2019;26(10):1142-1147
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the regulatory effect of miR-1297 on the malignant biological behaviors of breast cancer cells and its underlying mechanism. Methods: Twenty pairs of breast cancer tissues and para-cancer tissues resected at the Department of Thyroid and Breast Surgery of Leshan People′ s Hospital from May 2016 to May 2018, as well as breast cancer cell lines MCF-7, SW626, HCC1937 and human breast epithelial MCF-10A cells were collected for this study. qPCR was performed to evaluate the expression of miR-1297 in breast cancer tissues and cell lines. The experimental cells were divided into control group, miR-1297 inhibitor group; TET3 over-expression group and simultaneous over-expression of TET3 and miR-1297 group. CCK-8 assay was used to detect the cell proliferation of MCF-7 cells; Transwell assay was carried out to detect the migration and invasion of MCF-7 cells; and WB was used to measure the expressions of TET3 and EMT related proteins (E-cadherin, N-cadherin and vimentin). Dual luciferase reporter gene assay was used to verify the relationship between miR-1297 and TET3. Results: miR-1297 was up-regulated in both breast cancer tissues and cell lines (P<0.01 or P<0.05). Knockdown of miR-1297 dramatically repressed the proliferation, migration, invasion and EMT of MCF-7 cells (P<0.01 or P<0.05). Over-expression of TET3 significantly up-regulated the expression of TET3 in MCF-7 cells (P<0.05). Simultaneous over-expression of TET3 and miR-1297 could reverse the expression level of TET3 in MCF-7 cells and the inhibitory effect of TET3 on the proliferation, migration, invasion and EMT of MCF-7 cells. Dual luciferase reporter gene assay results showed that miR-1297 targetedly bound to the 3' UTR of TET3. Further experiment results demonstrated that miR-1297 targetedly down-regulated TET3 and promoted the malignant biological behaviors of MCF-7 cells. Conclusion: miR-1297 is up-regulated in breast cancer tissues and cells; it promotes the malignant biological behaviors such as proliferation, migration, invasion and EMT through targetedly down-regulating the expression of TET3.
- Full text:20191014.pdf
