Expression of Tspan29 in breast cancer tissues and its effect on malignant biological behaviors of MCF-7 and MDA-MB-231 cells
10.3872/j.issn.1007-385x.2020.01.007
- VernacularTitle:Tspan29在乳腺癌组织中的表达及其对MCF-7和MDA-MB-231细胞恶 性生物学行为的影响
- Author:
LI Gang
1
;
WANG Meixing
1
;
YANG Mei
2
;
DANG Xuefei
2
;
LI Xueqing
2
;
LI Xiaojing
2
;
WANG Hongxia
1
Author Information
1. Department of Oncology, Minhang Branch of Cancer Hospital Affiliated to Fudan University,
2. Department of Oncology, Songjiang Branch of the First People’s HospitalAffiliated to Shanghai JiaoTong University
- Publication Type:Journal Article
- Keywords:
breast cancer;
MCF-7 cell;
MDA-MB-231 cell;
tetraspanins-29 (Tspan29);
siRNA interference;
proliferation;
migration;
invasion;
epithelial-mesenchymal transition (EMT)
- From:
Chinese Journal of Cancer Biotherapy
2020;27(1):42-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of tetraspanins-29 (Tspan29) in breast cancer tissues and cell lines and to explore the effect of Tspan29 knockdown on proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) of breast cancer MCF-7 and MDA-MB-231 cells. Methods:Atotal of20pairsofbreast cancer tissues and corresponding para-cancerous tissues resected in Minhang Branch of Cancer HospitalAffiliated to Fudan University from June 2017 to February 2018 were collected for this study; in addition, breast cancer celllinesMCF-7,MDA-MB-231andhumanbreastepithelialMDA-kb2cellswerealsocollected.ThemRNAand protein expressions of Tspan29 in above mentioned tissues and cell lines were detected by Real-time quantitative (qPCR) and Western blotting. The expression of Tspan29 in MCF-7 and MDA-MB-231 cells was interfered by siRNA. qPCR was used to detect the mRNA and protein expressions of Tspan29. PCR microarray was used to examine the expressions of EMT-related genes in MCF-7 cells. CCK-8 assayandTranswellwereusedtodetectcellproliferation, migration and invasion of MCF-7 and MDA-MB-231 cells. Results: The mRNA and protein expressions of Tspan29 in breast cancer tissues were significantly higher than that in para-cancerous tissues (all P<0.01); and the mRNA and protein expressions of Tspan29 in MCF-7 and MDA-MB-231 cells were significantly higher than that in MDA-kb2 cells (P<0.01). After being interfered with siTspan29, the mRNA and protein expressions of Tspan29 were significantly down-regulated in MCF-7 cells (all P<0.05); the proliferation, invasion and migration of MCF-7 and MDA-MB-231 cells were significantly inhibited (all P<0.05); and among the EMT-related genes, two were significantly up-regulated while 7 were down-regulated. Conclusion: Tspan29 is significantly up-regulated in breast cancer tissues and cell lines, and knockdown of Tspan29 significantly inhibits the proliferation, invasion and migration of breast cancer cells.
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- Full text:20200107.pdf
