Effects of PTPN6 on malignant biological behaviors of human esophageal squamous cancer Eca109 andYes-2 cells
10.3872/j.issn.1007-385x.2019.03.004
- VernacularTitle:PTPN6基因对人食管鳞状细胞癌Eca109和Yes-2细胞恶性生物学行为的 影响
- Author:
LIU Lei
1
;
YANG Liu
2
;
NIU Yunfeng
2
;
LIANG Jia
2
;
SHEN Supeng
2
Author Information
1. Department of Thoracic Surgery, the Fourth Hospital of Hebei Medical University
2. Cancer Institute, the Fourth Hospital of Hebei Medical University
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
non-receptor protein tyrosine phosphatase 6 (PTPN6) gene;
Eca109 cell;
Yes-2 cell;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2019;26(3):273-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the expression of non-receptor protein tyrosine phosphatase 6 (PTPN6) in different esophageal squamous cell carcinoma (ESCC) cell lines, and to investigate its effect on proliferation, migration and invasion ability of Eca109 and Yes-2 cell lines. Methods: qPCR was applied to detect the mRNA expression of PTPN6 in different ESCC cell lines (TE1, Eca109, Kyse150, Kyse170 and Yes-2). pcDNA3.1-PTPN6 plasmid was transiently transfected into Eca109 and Yes-2 cells respectively. The expression of PTPN6 was detected by real-time PCR and Wb. The effects of PTPN6 over-expression on the biological behaviors of ESCC cells were detected by MTS, colony formation assay, wound healing assay and Transwell assay, respectively. Results: The mRNA expression of PTPN6 was remarkably reduced in ESCC cell lines (TE1, Eca109, Kyse150, Kyse170 and Yes-2) compared to normal esophageal epithelial cells (HEEpiC) (P<0.05). Compared to the mock cells, significant up-regulation of PTPN6 was detected in pcDNA3.1-PTPN6 transfected Eca109 and Yes-2 cells (all P<0.05 or P<0.01); PTPN6 over-expression led to a significant inhibition in migration and invasion ability of Eca109 and Yes-2 cells (all P<0.05). Conclusions: Over-expression of PTPN6 may inhibit the proliferation, migration and invasion of ESCC cells, which might be an important factor influencing the biological characteristics of ESCC cells.
- Full text:20190304.pdf
