Effect of electroacupuncture on expression of GABA receptor mRNA in different brain regions in rats with chronic inflammatory pain.
10.13703/j.0255-2930.20190129-k00054
- Author:
Jun-Ying DU
1
;
Zi-Tong XU
1
;
Fang-Bing SHAO
1
;
Ying-Ling XU
1
;
Xiao-Mei SHAO
1
;
Yi LIANG
1
;
Jian-Qiao FANG
1
;
Jun-Fan FANG
1
Author Information
1. Third College of Clinical Medicine, Zhejiang Chinese Medical University, Zhejiang Key Laboratory of Acupuncture Neurology, Hangzhou 310053, China.
- Publication Type:Journal Article
- Keywords:
GABAA receptor;
amygdala;
anterior cingulated cortex;
chronic inflammatory pain;
electroacupuncture (EA);
hypothalamus
- From:
Chinese Acupuncture & Moxibustion
2020;40(2):173-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the expression of GABA receptor mRNA in different brain regions of the central nervous system in chronic inflammatory pain rats and the intervention effect of electroacupuncture (EA).
METHODS:A total of 48 SPF male SD rats were randomly divided into a blank control group, a model control group, an EA group and a sham EA group, 12 rats in each group. The model of chronic inflammatory pain was established by injecting Freund's complete adjuvant into the foot. The EA group was treated with EA 28 days after the model establishment. The "Housanli" (ST 36) and "Kunlun" (BL 60) were selected and treated with dilatational wave, 2 Hz/100 Hz in frequency, 0.5-1.5 mA for 30 min; EA was given only once. In the sham EA group, the same acupoints were selected but the needles were only inserted into subcutaneous area; EA was connected for 30 min without electrical stimulation. The behavior changes of mechanical pain threshold and thermal pain threshold before model establishment, 1 day, 3 days, 7 days, 14 days, 21 days and 28 days after the model establishment as well as emotional behavior 29 days after the model establishment were observed; the relative expressions of GABA receptor mRNA in anterior cingulate cortex, amygdala and hypothalamus were observed.
RESULTS:Compared with the blank control group, the change rates of mechanical pain threshold and thermal pain threshold in the model control group were decreased significantly 1 day, 3 days, 7 days, 14 days, 21 days, 28 days after model establishment (<0.01); 29 days after model establishment, the movement distance and staying time in the central area of open field test in the model control group were decreased significantly (<0.05). After EA intervention, compared with the model control group and the sham EA group, the change rates of mechanical pain threshold and thermal pain threshold, as well as the movement distance and the staying time of central area were significantly increased in the EA group (<0.01, <0.05). Twenty-nine days after model establishment, the expression of GABA receptor mRNA in anterior cingulate cortex and hypothalamus was not significantly different among all groups (>0.05). Compared with the blank control group, the expression of GABA receptor mRNA in the amygdala was decreased significantly in the model control group (<0.01); compared with the model control group and the sham EA group, the expression of GABA receptor mRNA in amygdala was increased after intervention in the EA group (<0.01).
CONCLUSION:Single treatment of EA could significantly increase the mechanical pain threshold and thermal pain threshold, improve abnormal emotional behavior in rats with chronic inflammatory pain, which may be related to the increasing of expression of GABA receptor mRNA in the amygdala.
