Urine Metabonomic Analysis of Interventions Effect of Soy Isoflavones on Rats Exposed to Di-(2-ethylhexyl) Phthalate.
- Author:
Xin Wen DONG
1
;
San Qiao YAO
2
;
Hao Yu WU
3
;
Yun Bo ZHANG
3
;
Cheng WANG
3
;
Xiao Lin NA
3
;
Wei Dong WU
2
Author Information
- Publication Type:Journal Article
- Keywords: Di-(2-ethylhexyl) phthalate; Metabonomics; Soy isoflavones; UPLC-QTOF-MS/MS; Urine
- From: Biomedical and Environmental Sciences 2020;33(2):77-88
- CountryChina
- Language:English
-
Abstract:
Objective:Di-(2-ethylhexyl) phthalate (DEHP) is a ubiquitous environmental contaminant. As an endocrine disruptor, it seriously threatens human health and ecological environmental safety. This study examines the impact of intervention with soybean isoflavones (SIF) on DEHP-induced toxicity using a metabonomics approach.
Methods:Rats were randomly divided into control (H), SIF-treated (A, 86 mg/kg body weight), DEHP-treated (B, 68 mg/kg), and SIF plus DEHP-treated (D) groups. Rats were given SIF and DEHP daily through diet and gavage, respectively. After 30 d of treatment, rat urine was tested using UPLC/MS with multivariate analysis. Metabolic changes were also evaluated using biochemical assays.
Results:Metabolomics analyses revealed that p-cresol glucuronide, methyl hippuric acid, N1-methyl-2-pyridone-5-carboxamide, lysophosphatidycholine [18:2 (9Z, 12Z)] {lysoPC [18:2 (9Z, 12Z)]}, lysoPC (16:0), xanthosine, undecanedioic acid, and N6-acetyl-l-lysine were present at significantly different levels in control and treatment groups.
Conclusion:SIF supplementation partially protects rats from DEHP-induced metabolic abnormalities by regulating fatty acid metabolism, antioxidant defense system, amino acid metabolism, and is also involved in the protection of mitochondria.
