Effect of propofol on HMGB1/TLR4 signaling pathway during hepatic ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2019.07.026
- VernacularTitle:异丙酚对大鼠肝缺血再灌注损伤时HMGB-1/TLR4信号通路的影响
- Author:
Shuzhen YU
1
;
Weiwei ZHANG
;
Junming REN
;
Jianfeng WEI
;
Yu ZHANG
;
Lina ZHENG
;
Lijun HAO
;
Yuehong QI
;
Tiane LUO
;
Yongqing GUO
Author Information
1. 山西省人民医院麻醉科
- Keywords:
Propofol;
Liver;
Reperfusion injury;
High mobility group proteins;
Toll-like receptor 4
- From:
Chinese Journal of Anesthesiology
2019;39(7):870-872
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of propofol on high-mobility group box 1 protein (HMGB1)/Toll-like receptor 4 (TLR4) signaling pathway during hepatic ischemia-reperfusion (I/R) injury in rats.Methods Thirty-six clean-grade healthy male Sprague-Dawley rats,aged 3 months,weighing 250 -300 g,were divided into 3 groups (n=12 each) using a random number table method:sham operation group (group S),hepatic I/R group (group I/R) and propofol group (group P).Hepatic I/R injury was induced by occluding the portal vein and hepatic artery supplying the left and middle lobes of the liver for 1 h followed by 6-h reperfusion in anesthetized rats.Propofol was infused via the tail vein at a rate of 12 mg ·kg-1 · h-1 starting from 20 min before ischemia until 6 h of reperfusion in group P.The rats were sacrificed at 6 h of reperfusion,and the left lobe of the liver was removed for microscopic examination of the pathological changes which were scored and for determination of the expression of HMGB1,TLR4,tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-6) in liver tissues (by Western blot).Results Compared with group S,pathological scores of liver tissues were significantly increased,and the expression of HMGB1,TLR4,TNF-α and IL-6 was up-regulated in I/R and P groups (P＜0.05).Compared with group I/R,pathological scores of liver tissues were significantly decreased,and the expression of HMGB1,TLR4,TNF-α and IL-6 was down-regulated in group P (P＜ 0.05).Conclusion The mechanism by which propofol reduces liver I/R injury is associated with blocking HMGB-1/TLR4 signaling pathway and inhibiting inflammatory responses in rats.
