Study on improving the quality standard of Taohong Tongmai granule

VernacularTitle:桃红通脉颗粒质量标准提高研究
Author: Weihua JIN ¹ ; Hua CHEN ; Ming ZHANG ; Botao YU ; Zhiqiang PU ; Zonghui SONG
Author Information

1. 原成都军区总医院药剂科
Keywords: Taohong Tongmai granule; TLC; HPLC; Paeoniflorin
From: Journal of Pharmaceutical Practice 2018;36(2):162-165,169
CountryChina
Language:Chinese
Abstract: Objective To improve the quality standard of Taohong Tongmai granule.Methods TLC was used to make qualitative identification of Paeoniae Radix Rubra,Rehmanniae Radix,Glycyrrhizae Radix;The effective components of the main walnut kernel were identified by HPLC;and HPLC was used to do quantitative determination of Paeoniflorin,the determination was performed on Agilent HC-C18(250mm×4.6mm,5μm)column with mobile phase consisted of Acetonitrile-water(13:87) at the flow rate of 1.0 ml/min,the column temperature was 30 ℃ and the detection wavelength was set at 230 nm.Results These TLC spots were fairly clear,and the blank test showed no interference;The paeoniflorin at the range of 1·79-57.28 μg/ml was linear with peak area(r= 0.999 9),its average recovery rate was 99.99% and RSD was 2.05%(n= 9). Conclusion The quality standard of Taohong Tongmai granule had been improved,identification method was better reproduc-ibility,the determination method of paeoniflorin content had enhanced the controllability of product quality.