Simultaneous determination of dexamethasone acetate , camphor and phenol in compound cream by HPLC method

VernacularTitle:HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚的含量
Author: Yali WANG ¹ ; Xiandong PENG ; Weiyi HAO ; Dan WANG ; Tingdong GUO ; Zhili TANG
Author Information

1. 南充市中心医院药学部
Keywords: HPLC; cream; dexamethasone acetate; camphor; phenol; determination
From: Journal of Pharmaceutical Practice 2017;35(6):551-553,576
CountryChina
Language:Chinese
Abstract: Objective To establish a HPLC method for simultaneously determining the content of dexamethasone ace-tate ,camphor and phenol in compound cream .Methods The separation was performed on a SHIADZU-GL Inertsil? ODS-3 RP C18 analytical column with the mobile phase consisting of methanol-water (60:40) .The flow rate was 1 .0 ml/min .The detection wave length was 285 nm and the column temperature was 40 ℃ .Results Dexamethasone acetate ,camphor and phe-nol showed good linearity (r> 0 .9995 , n= 7) within the concentration range of 4 .024-40 .24 ,101 .7-2033 and 10 .38-425 .2 μg/ml ,respectively . The average recovery of dexamethasone acetate ,camphor and phenol was 101 .2％ (RSD was 0.56％ ) ,99 .89％ (RSD was 0 .72％ ) ,100 .2％ (RSD was 0 .97％ ) ,respectively .Moreover ,the RSDs were less than 1 .5％ in the repeated tests .Conclusion The method was simple ,quick and accurate .It is suitable for the quality control of dexametha-sone acetate camphor and phenol cream .