Genotoxicity evaluation of triptolide
10.3969/j.issn.1006-0111.2016.03.006
- VernacularTitle:雷公藤内酯醇的遗传毒性评价
- Author:
Yijun TIAN
1
;
Yiwen ZHENG
;
Yuping ZHU
;
Xiaofang ZHANG
;
Ying ZONG
;
Guocai LU
Author Information
1. 第二军医大学卫生毒理学教研室
- Keywords:
triptolide;
mutagenicity test;
Ames test;
chromosomal aberration test;
micronucleus assay
- From:
Journal of Pharmaceutical Practice
2016;34(3):215-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genotoxicity of triptolide ,an important active component of Tripterygium wilfordii Hook f .Methods Ames test ,in vitro chromosomal aberration test of CHO cell and in vivo micronucleus assay were per-formed to investigate the genotoxicity of triptolide .Results The Ames test showed that triptolide did not increase mutagenicity for TA97 ,TA98 ,TA100 ,TA102 and TA1535 strains at the dosage of 1 .6~1000 μg per plate with and without metabolic ac-tivation system S9 .Results of in vitro CHO cell chromosomal aberration test indicated that there was no statistical difference between the triptolide groups (doses of 0 .01 ,0 .02 and 0 .04 μg/ml) and the solvent control group with and without metabolic activation system S9 .However ,triptolide significantly increased polychromatophilic erythrocyte micronucleus formation at the dosage of 720 μg/kg in ICR mice .Conclusion Triptolide did not induce genetic toxicity based on the Ames test and chromo-somal aberration test ,but could increase micronucleus formation at the dosage of 720 μg/kg .These results indicated that trip-tolide may have potential genotoxicity on human health .
