Determination of asiatic acid in the root of Actinidia valvata by HPLC
10.3969/j.issn.1006-0111.2015.04.017
- VernacularTitle:应用高效液相色谱法测定猫人参中积雪草酸的含量
- Author:
Fang LÜ
1
;
Jin ZHOU
;
Yang FEI
;
Yu CHEN
;
Guoqing ZHANG
;
Liang ZHAO
Author Information
1. 第二军医大学附属东方肝胆外科医院药材科
- Keywords:
Actinidia valvata;
asiatic acid;
determination;
HPLC
- From:
Journal of Pharmaceutical Practice
2015;(4):356-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of asiatic acid in the root of Actinidia valvata . Methods HPLC-VWD was used in the analysis .The column was Agilent HC-C18 (4 .6 mm × 250 mm ,5μm);the mobile phase was acetonitrile :(30 mmol/L) acetic acid amine solution (35:65);the flow rate was 1 .0 ml/min;the temperature of column was 25 ℃ ;the detection wavelength was set at 210 nm ;the injection volume was 25 μl;the running time was 35 min .Results
Asiatic acid was separated with interference in baseline .The linear range was 25 .30-506 .0 μg/ml with linear correlation of 0 .999 6 for asiatic acid .The result of intra-day and inter-day precisions were both within 5% (n=3) ,and the average recovery was 99.4% with RSD 1 .9% (n=6) .Conclusion The method was simple ,rapid ,accurate and convenient for quality control of asiatic acid in the root of Actinidia valvata .
