Screening for the hemostatic active extracts of Callicarpa nudi f lora,in mice in vivo
10.3969/j.issn.1006-0111.2015.03.011
- VernacularTitle:黎药裸花紫珠在小鼠体内止血活性部位的研究
- Author:
Bo YI
1
;
Min ZHANG
;
Hai LIN
;
Tao CHEN
;
Wentong XU
;
Shixiu FENG
Author Information
1. 解放军187医院药剂科
- Keywords:
Callicarpa nudif lora;
active extracts;
bleeding time;
clotting time;
mice
- From:
Journal of Pharmaceutical Practice
2015;(3):235-237,241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the hemostatic active extracts from Callicarpa nudif lora .Methods The powdered Callicarpanudiflora was extracted with 70% EtOH and concentrated to give EtOH-extract .The EtOH-extract was further chromatographed over HP-20 macroporous resin column ,eluting with aqua and 95% EtOH to get HP-H2 O-elution and HP-EtOH-elution ,respectively .The obtained three extracts of EtOH-extrract ,HP-EtOH-elution and HP-H2 O-elution were set as large ,middle and small dosage groups for drug preparation ,respectively .Yunnan Baiyao was used as a positive control group . The weight increment ,bleeding time and clotting time of fed mice were detected by cutting tail and grass slide methods after in-tragastric administration for 7 days .Results As compared with blank model and positive control ,each dosage groups of HP-EtOH-elution could significantly shortened the bleeding time ,of which the small dosage group and middle dosage group even ex-hibited better results than the positive control group .Whereas the EtOH-extract ,HP-EtOH-elution and HP-H2 O-elution didn′t demonstrated significant effect on clotting time as well as the weight increment .Conclusion The HP-EtOH-elution was suggested to be the major active extract possessing hemostatic activity to mice under tested dosages .The hemostatic mechanism may through in-trinsic coagulation pathway .This study would be helpful for further phytochemical investigation of C .nudi f lora .
