The expression characteristics and significance of peripheral blood lymphocyte programmed death-1 in patients with aneurysmal subarachnoid hemorrhage
10.3969/j.issn.1004-1648.2019.05.022
- VernacularTitle:动脉瘤蛛网膜下腔出血患者外周血淋巴细胞程序性死亡受体1表达特性及意义的研究
- Author:
Yi GAO
1
;
Yuhang AI
Author Information
1. 410200长沙康乃馨老年病医院重症医学科
- Keywords:
aneurysm;
subarachnoid hemorrhage;
programmed death-1;
CD4 + cells;
CD8 + cells
- From:
Journal of Clinical Neurology
2019;32(5):368-371
- CountryChina
- Language:Chinese
-
Abstract:
By observing the expression of peripheral blood lymphocyte programmed death-1 (PD-1) in patients with aneurysm subarachnoid hemorrhage (aSAH), the expression characteristics and significance in patients with aSAH were explored. Methods A total of 21 patients with aSAH were selected and were divided into neurological symptoms group [ Glaskow Coma Scale ( GCS) <15, 10 cases] and non-neurological symptoms group (GCS=15, 11 cases) according to GCS. And 6 health human were selected as the control group. The expression of CD4 + and CD8 + T lymphocytes in peripheral blood of patients in each group was detected by flow cytometry, and its characteristics and significance were analyzed. Results The percentages of PD-1 + CD4 + T lymphocyte and PD-1 +CD8 + T lymphocytein of non-neurological symptoms group were significantly higher than those in control group, while significantly lower than those in neurological symptoms group ( all P< 0.05 ). The percentages of PD-1 +CD4 + T lymphocyte and PD-1 +CD8 + T lymphocytein were significantly correlated with the GCS score, and the Spearson coefficients were 0.87 and 0.83, respectively (all P<0.05). In the aSAH patients, the incidence of pulmonary infection in neurological symptoms group was significantly higher than that in non-neurological symptoms group (P<0.05), and the percentages of PD-1 +CD4 + T lymphocyte and PD-1 +CD8 + T lymphocytein in pulmonary infection group was significantly higher than that in non-pulmonary infection group ( all P< 0.05 ). Conclusion In patients with aSAH, especially those with neurological symptoms, the expression intensity of CD4 +and CD8 + T lymphocyte PD-1 is significantly increased, which is correlated with the severity of neurological symptoms after aSAH and pulmonary infection after hemorrhage.
