Construction of a concentration gradient microfluidic chip platform for the rapid screening of drugs active against Candida albicans
10.16438/j.0513-4870.2019-0770
- VernacularTitle:浓度梯度微流控芯片平台的构建及其应用于抗白念珠菌药物快速筛选研究
- Author:
Ying CAI
1
;
Yang CHEN
2
;
Zhan-ying HONG
1
;
Yi-feng CHAI
3
Author Information
1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; School of Pharmacy, Naval Medical University, Shanghai 200433, China
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China; Drug Instrument Supervision and Inspection Station, Xining Joint Logistics Support Center, Lanzhou 730050, China
3. School of Pharmacy, Naval Medical University, Shanghai 200433, China
- Publication Type:Research Article
- Keywords:
microfluidic chip;
concentration gradient generation;
characterization;
rug screening;
italic>Candida albicans
- From:
Acta Pharmaceutica Sinica
2020;55(2):323-329
- CountryChina
- Language:Chinese
-
Abstract:
Drug screening against Candida albicans has become more urgent due to the increasing incidence of infection and the development of drug-resistant strains. The microfluidic chip technique has shown great potential for high-throughput drug screening. In this study we developed a concentration gradient microfluidic chip platform for drug screening against Candida albicans. The generated concentration gradient on this platform was investigated qualitatively by monitoring the distribution of the fluorescent tracer fluorescein sodium and quantitatively by following the distribution of the model drug fluconazole as analyzed by HPLC; the effect of different flow conditions on the concentration gradient were determined. The ratio of the two aqueous phase flow rates was determined in the subsequent drug screening studies. Alamar Blue, an indicator of cell viability, was used in the susceptibility test for amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, terbinafine, 5-fluorocytosine and caspofungin as carried out on the established chip platform. The MIC range of the drugs, which was consistent with the MIC values of the CLSI-recommended standard, were obtained quickly and efficiently through the use of this platform, indicating that this new platform can quickly screen a series of antibacterial drugs in one run. In addition, the strain of Candida albicans we used showed resistance to terbinafine in our platform assay, consistent with the results of a 96-well plate assay, indicating that the platform can also be used for rapid screening of resistant strains.
