Massive Fetomaternal Hemorrhage Diagnosed with High-performance Liquid Chromatography
10.15264/cpho.2016.23.2.158
- Author:
Ji Young LEE
1
;
Kyoung Ha KIM
;
Seom Gim KONG
Author Information
1. Department of Pediatrics, Kosin University Gospel Hospital, Kosin University College of Medicine, Busan, Korea. ana313@hanmail.net
- Publication Type:Case Report
- Keywords:
Fetomaternal hemorrhage;
HPLC;
Fetal hemoglobin
- MeSH:
Anemia, Neonatal;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Diagnosis;
Female;
Fetal Death;
Fetal Hemoglobin;
Fetomaternal Transfusion;
Flow Cytometry;
Hemoglobin A, Glycosylated;
Humans;
Infant, Newborn;
Pallor;
Pregnancy;
Prothrombin Time
- From:Clinical Pediatric Hematology-Oncology
2016;23(2):158-161
- CountryRepublic of Korea
- Language:English
-
Abstract:
Massive fetomaternal hemorrhage (FMH) is a major cause of unexplained fetal death and neonatal anemia. FMH can be diagnosed using the Kleihauer-Betke test or flow cytometry by identifying the presence of fetal red cells in the maternal blood. However, timely diagnosis is a challenge because many hospitals lack the equipment needed to perform such tests. The authors experienced a case of FMH diagnosed via high-performance liquid chromatography (HPLC) which is generally used in measuring glycated hemoglobin (HbA1c) in a patient with unexplained neonatal anemia. A girl aged 2 days was transferred to our hospital for showing pallor and a hemoglobin level of 5.0 g/dL. HPLC revealed 3% fetal hemoglobin (HbF) in the maternal blood. HPLC is a quick test for quantifying HbF that is readily available in many hospitals and could serve as a promising alternative for diagnosing FMH.
