Anti-inflammatory and Immunosuppressive Effects of Panax notoginseng
10.20307/nps.2019.25.4.317
- Author:
Thao Quyen CAO
1
;
Jae Hyuk HAN
;
Hyun Su LEE
;
Manh Tuan HA
;
Mi Hee WOO
;
Byung Sun MIN
Author Information
1. College of Pharmacy, Drug Research and Development Center, Daegu Catholic University, Gyeongbuk 38430, Republic of Korea. bsmin@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Panax notoginseng;
anti-inflammation;
immunosuppressive;
ginsenoside;
polyacetylene;
saponin
- MeSH:
Ginsenosides;
Interleukin-2;
Nitric Oxide;
Panax notoginseng;
Panax;
Plants;
Polyacetylenes;
Saponins;
T-Lymphocytes
- From:Natural Product Sciences
2019;25(4):317-325
- CountryRepublic of Korea
- Language:English
-
Abstract:
Here, we designed to examine the anti-inflammatory effects on RAW264.7 cells and the immunosuppressive effects by evaluating interleukin-2 (IL-2) production in Jurkat T cells using a MeOH extract of Panax notoginseng roots. The results showed that the MeOH extract inhibited the synthesis of nitric oxide (NO) in a dose-dependent manner (IC₅₀ value of 7.08 µg/mL) and displayed effects on T cell activation at a concentration of 400 µg/mL. In efforts to identify the potent compounds, bioactivity-guided fractionation of the MeOH extract and chemical investigation of its active CH₂Cl₂-, EtOAc-, and butanol-soluble fractions led to the successful isolation and identification of eleven compounds, including two polyacetylenes (1, 2), a steroid saponin (3), seven dammarane-type ginsenosides (4 – 10), and an oleanane-type ginsenoside (11). Among them, compound 11 was isolated from this plant for the first time. Compound 2 exhibited potent inhibitory effects on NO synthesis and an immunosuppressive effect with IC₅₀ values of 2.28 and 65.57 µM, respectively.
