Identification of Candida Species Using 26S Ribosomal RNA Gene Sequencing in Patients with Periodontitis
10.4167/jbv.2019.49.4.212
- Author:
Nebras Nasir AL-DABBAGH
1
;
Wissam Hamid AL-JANABI
;
Mohammed Baqur Sahib AL-SHUHAIB
Author Information
1. Department of Microbiology, College of Dentistry, University of Babylon, Babil 51001, Iraq.
- Publication Type:Original Article
- Keywords:
26S ribosomal RNA;
Candida;
infection;
DNA sequence analysis;
Oral cavity
- MeSH:
Candida;
Chronic Periodontitis;
Dentists;
Discrimination (Psychology);
DNA;
Genes, rRNA;
Humans;
Mass Screening;
Mouth;
Periodontitis;
Polymerase Chain Reaction;
Prevalence;
RNA, Ribosomal;
Sequence Analysis, DNA
- From:Journal of Bacteriology and Virology
2019;49(4):212-220
- CountryRepublic of Korea
- Language:English
-
Abstract:
The infection with Candida spp. for oral cavity is being increasingly reported. However, its variations have not yet been specifically described in periodontitis. The present study was conducted to use an uniplex 26S rRNA-based amplicons to detect and discriminate Candida using only one pair of ribosomal primers. A total of 50 patients with chronic periodontitis was involved in the study. Pure Candida colonies were isolated from 23 patients and genomic DNA was extracted, and PCR was conducted. Direct DNA sequencing followed by comprehensive phylogenetic analyses were performed to confirm the identity of Candida colonies. Results indicated that the ration of Candida-infected patients was 46%, with a high prevalence of C. albicans, followed by remarkably lower ratios of C. parapsilosis, C. glabrata, C. kefyr, and C. dubliniensis respectively. Phylogenetic analyses indicated obvious discrimination amongst the analyzed Candida species as each observed species occupied a distinctive phylogenetic position. The current results reported a simple, efficient, and low-cost detection of five species of Candida without the need for other costly techniques of molecular screening. The current findings may help dentists to easily take a snapshot of the patterns of Candida infection in periodontitis cases to assess the nature and grade of infection.
