Development of an Internally Controlled Reverse Transcription Recombinase-aided Amplification Assay for the Rapid and Visual Detection of West Nile Virus.

Guo Hao FAN; Xin Xin SHEN; Fan LI; Xin Na LI; Xue Ding BAI; Rui Qing ZHANG; Rui Huan WANG; Wen Wen LEI; Huan Yu WANG; Xue Jun MA; Gui Zhen WU

Return

Development of an Internally Controlled Reverse Transcription Recombinase-aided Amplification Assay for the Rapid and Visual Detection of West Nile Virus.

Author: Guo Hao FAN ¹ ; Xin Xin SHEN ² ; Fan LI ³ ; Xin Na LI ² ; Xue Ding BAI ² ; Rui Qing ZHANG ² ; Rui Huan WANG ² ; Wen Wen LEI ³ ; Huan Yu WANG ³ ; Xue Jun MA ⁴ ; Gui Zhen WU ⁵
Author Information

1. Department of Core Facility, Ministry of Health Key Laboratory of Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Biosafety 3 Laboratory, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. Department of Core Facility, Ministry of Health Key Laboratory of Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
3. State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Department of Viral Encephalitis, NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
4. Department of Core Facility, Ministry of Health Key Laboratory of Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
5. Biosafety 3 Laboratory, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Publication Type:Journal Article
Keywords: Detection; Lateral flow dipstick (LFD); RT-RAA; WNV
From: Biomedical and Environmental Sciences 2019;32(12):926-929
CountryChina
Language:English
Abstract: West Nile virus (WNV) causes West Nile fever and West Nile encephalitis. Because infection by WNV creates serious public health problems, its simple, rapid, and visual detection is very important in clinical practice, especially in resource-limited laboratories. We have developed a rapid, specific, and highly sensitive internally controlled reverse transcription recombinase-aided amplification (RTRAA) assay to detect WNV, using both real-time fluorescence and the lateral flow dipstick (LFD) at 39.0 °C for 30 min. The analytical sensitivity of the RT-RAA assay was 10 plasmid copies and 1.6 pfu per reaction with real-time fluorescence, and 1,000 plasmid copies per reaction with the LFD. No crossreaction with other control viruses was observed. Compared with the RT-qPCR assay, the RT-RAA assay demonstrated 100% sensitivity and 100% specificity for WNV.