BAX Gene Deletion Reduces the Sensitivity of BCR-ABL-Induced B-ALL Cells of Mice to Imatinib.
10.7534/j.issn.1009-2137.2019.06.007
- Author:
Liang SHI
1
;
Yuan-Yuan LONG
1
;
Xi LUO
1
;
Qiong SU
1
;
Pei HUANG
1
;
Sheng-Sen YAO
1
;
Yan CHEN
2
,
3
Author Information
1. Second Department of Pediatrics, Zunyi Medical University，Affiliated Hospital of Zunyi Medical University，The Children's Hospital of Guizhou Province，Zunyi 563003，Guizhou Province，China.
2. Second Department of Pediatrics, Zunyi Medical University，Affiliated Hospital of Zunyi Medical University，The Children's Hospital of Guizhou Province，Zunyi 563003，Guizhou Province，China,E-mail: cyz600@
3. com.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Drug Resistance, Neoplasm;
Fusion Proteins, bcr-abl;
Gene Deletion;
Imatinib Mesylate;
Mice;
Piperazines;
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
bcl-2-Associated X Protein
- From:
Journal of Experimental Hematology
2019;27(6):1749-1753
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of BAX gene deletion on the sensitivity of BCR-ABL-induced B-ALL cells of mice to imatinib and the related mechanism.
METHODS:The target gene-knock out (BAX) mice were used as bone marrow cell donors; the wild type bone marrow cells(B6BM) and BAX bone marrow cells(B6BM-BAX) of mice were transfected by using reverse transcription virus, then the BCR-ABL transfected B6BM cells and B6BM-BAX cells were treated with imatinib at different concentration (0,0.5, 1.0 and 2.0 μmol/L) for 48 hours. The number of viable cells was detected by trypan blue, the flow cytometry was used to detect the cell apoptosis, the Western blot was used to detect the changes of BAX, Caspase expression.
RESULTS:In BCR-ABL transfected bone marrow cells treated with imatinib, the numbers of viable cells of BAX deletion group was significantly higher than that of wild type groups with statristcal difference(P<0.05), and effect- and dose-dependency(r=-0.9533 for BAX deletion group, and r=-0.9812 for wild type group). The flow cytometry showed that the cell apoptosis in BAX deletion group signifincantly decreased, compared with wild type group(P<0.05). The Western blot showed that the expression of apoptotic protein Caspase 3 in BAX deletion group was significantly higher than that in wild type group(P<0.05).
CONCLUSION:BAX deletion can reduce the sensitivity of BCR-ABL-induced B-ALL cells to imatinib.
