Role of protein kinase D1 in regulating the growth, apoptosis and drug sensitivity of oral squamous carcinoma cells.
- Author:
Jing-Nan WANG
1
;
Ya-Ping FAN
1
;
Jiao CHEN
1
;
Yun FENG
1
;
Bo-Miao CUI
1
;
Xiao-Ying LI
1
;
Li-Wei WANG
1
;
Hong-Li CHEN
1
;
Ping ZHANG
1
;
Hong-Kun WU
1
Author Information
- Publication Type:Journal Article
- Keywords: multi-drug resistance; oral squamous carcinoma; paclitaxel; protein kinases D1
- MeSH: Apoptosis; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Proliferation; Humans; Mouth Neoplasms
- From: West China Journal of Stomatology 2019;37(6):583-588
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:This study aimed to investigate the role of protein kinase D (PKD)1 in regulating the growth, apop-tosis, and drug sensitivity of the squamous carcinoma cell line SCC-25.
METHODS:The SCC-25 cell line was transfected with either the control-shRNA or PKD1-shRNA plasmids. The stable transfected cells were selected, and the efficiency of PKD1 knockdown was detected by Western blot. The growth and apoptosis of SCC-25 were analyzed with a cell counting kit-8 (CCK8) and flow cytometry. The 50% inhibitory concentrations (IC50) of paclitaxel in the control and PKD1 knockdown cell lines were detected by CCK-8. The expression levels of Bax, Bcl-2, and P-gp were detected by Western blot.
RESULTS:PKD1 was constitutively expressed and phosphorylated in various cancer cell lines. Inhibiting the expression of PKD1 in SCC-25 cells by RNA interference could inhibit the growth and promote the apoptosis of SCC-25 cells via downregulating Bcl-2 expression. Additionally, inhibiting PKD1 expression could downregulate the expression of P-gp, thereby decreasing both the IC50 and resistance index of paclitaxel.
CONCLUSIONS:PKD1 plays an important role in regulating the biobehavior of SCC-25. It is a potential therapeutic target for oral squamous carcinoma.
